Merck
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4370285

Sigma-Aldrich

Trypsin, TPCK-Treated

All Photos(1)
Synonym(s):
Trypsin, TPCK treated for Cell Culture
EC Number:
232-650-8
NACRES:
NA.56

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Trypsin from bovine pancreas TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

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T1426

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TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

Trypsin from bovine pancreas TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

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T8802

Trypsin from bovine pancreas

TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

Trypsin from porcine pancreas lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid

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T4799

Trypsin from porcine pancreas

lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid

Trypsin Acetylated from bovine pancreas Type V-S, ≥8,500 BAEE units/mg protein (biuret)

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T6763

Trypsin Acetylated from bovine pancreas

Type V-S, ≥8,500 BAEE units/mg protein (biuret)

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T6567

Trypsin from porcine pancreas

Proteomics Grade, BioReagent, Dimethylated

Trypsin from bovine pancreas suitable for protein sequencing, lyophilized powder

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T8658

Trypsin from bovine pancreas

suitable for protein sequencing, lyophilized powder

vibrant-m

T2600000

Trypsin

BRP, European Pharmacopoeia (EP) Reference Standard

N-p-Tosyl-L-phenylalanine chloromethyl ketone ≥97% (TLC), powder

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T4376

N-p-Tosyl-L-phenylalanine chloromethyl ketone

≥97% (TLC), powder

Trypsin from porcine pancreas Type IX-S, lyophilized powder, 13,000-20,000 BAEE units/mg protein

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T0303

Trypsin from porcine pancreas

Type IX-S, lyophilized powder, 13,000-20,000 BAEE units/mg protein

Trypsin from bovine pancreas Type I, ~10,000 BAEE units/mg protein

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T8003

Trypsin from bovine pancreas

Type I, ~10,000 BAEE units/mg protein

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Trypsin is a serine protease that specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. This modified trypsin has been treated with N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) to inactivate extraneous chymotryptic activity. Each package contains 8 vials, with 25 μg in each vial.

Application

Trypsin, TPCK-Treated has been used:
  • as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) for porcine delta coronavirus (PDCoV) infection experiments using epithelial-like pig kidney cell line (LLC-PK1)
  • to detach the human umbilical vein endothelial cells (HUVEC) for annexin-V/propidium Iodide (PI) staining assay
  • in minimum essential medium (MEM) for multicycle replication kinetics

Packaging

1 kit

Biochem/physiol Actions

N-p-Tosyl-L-phenylalanine chloromethyl ketone (TPCK) serves as an irreversible inhibitor of chymotrypsin. Trypsin induces human fibrocyte differentiation. Trypsin is widely used in proteomics for protein sample digestion. In cell culture, trypsinization is carried out to dislodge adherent cells from each other and the walls of the culture vessel.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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