59429C

Trypsin (1X)

sterile; γ-irradiated, 0.25% porcine trypsin (1:250) in HBSS, w/0.1% EDTA-Na₂, w/o Ca⁺⁺ and Mg⁺⁺

• Synonym(s): Trypsin-EDTA solution
• MDL number: MFCD00130286
• NACRES: NA.75

Properties

• Quality Level: 500
• description: for research or for further manufacturing use
• sterility: sterile; γ-irradiated
• form: liquid
• technique(s): cell culture | mammalian: suitable
• components: phenol red: 10.620 mg/L; NaHCO₃: 350.000 mg/L
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. They are generally composed of variable concentrations of natural porcine pancreas-derived trypsin and EDTA. Trypsin 1X solutions can range from 0.025% to 0.5%. The reasons for the range of concentrations are differences in required trypsin activity or potency; differences in incubation times; and cell line susceptibility. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.

Preparation Note

SAFC Biosciences has validated a process using γ radiation to significantly reduce the risks associated with adventitious agents such as Porcine Parvovirus (PPV), PorcineRespiratory and Reproductive Syndrome (PRRS) and Mycoplasma hyorhinis while maintaining product performance. The use of γ irradiated trypsin requires no change to the end user′s methods or procedures while giving additional assurance against microbial contaminants associated with animal-derived products. All trypsin is obtained from the United States or other countries deemed free of Bovine Spongiform Encephalopathy (BSE).

This product does contain phenol red. Due to shipment on dry ice, there could be significant carbon dioxide buildup in the package. This CO₂ may enter the solution and lower the pH slightly, giving an orange rather than pinkish color. The orange solution will still be suitable for use, or the pH can be adjusted with sodium hydroxide. Incubating cells with too high a trypsin concentration for a long period can damage cell membranes and kill the cells. Solubilizing trypsin or diluting it from a concentrated solution should be done with a buffered salt solution containing no Ca²⁺ or Mg²⁺.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable