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806552

SAFC

Phosphate Buffered Saline

pH 7.4, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
Phosphate buffered saline, PBS
MDL number:
MFCD00131855
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

200

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

pH

7.4

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This Item
806544P4417D1408
SAFC

SAFC

806552

Phosphate Buffered Saline

SAFC

SAFC

806544

Phosphate Buffered Saline

Phosphate buffered saline tablet

Sigma-Aldrich

P4417

Phosphate buffered saline

Sigma-Aldrich

Sigma-Aldrich

D1408

Dulbecco′s Phosphate Buffered Saline

form

liquid

form

liquid

form

tablet

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

impurities

Endotoxin, tested

impurities

-

impurities

endotoxin, tested

pH

7.4

pH

7.2

pH

7.2-7.6 (1 tablet/200 mL)

pH

-

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

400

General description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

Application

Phosphate-buffered saline has been used:
  • as a reagent in the isolation of human endometrial stem cell/stromal cells (hEnSCs) from the endometrium or menstrual blood
  • has also been used to wash cells during immunofluorescence staining
  • as a solvent for the glucose administered orally in the Glucose Tolerance Tests (GTTs)

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Human Endometrial Stem Cell Isolation from Endometrium and Menstrual Blood
Tavakol S, et al.
Molecular Neurobiology (2016)
Dieter Fink et al.
PloS one, 14(11), e0224576-e0224576 (2019-11-08)
Intra-bone marrow transplantation (IBMT) has been adapted for mouse models to improve the seeding efficiency of transplanted hematopoietic stem and progenitor cells. Commonly used injection volumes for IBMT into the tibia differ between 10 and 40 μL even though considerable
L R Gabbert et al.
Journal of applied microbiology, 129(5), 1092-1101 (2020-05-08)
Develop an effective laboratory method to consistently recover viral loads from porous concrete coupons sufficient for disinfectant efficacy testing. Investigate the role of concrete matrix pH on the recovery of foot-and-mouth disease virus (FMDV) and African Swine Fever virus (ASFV)
Pinkal D Patel et al.
Molecular vision, 26, 434-444 (2020-06-23)
Chronic elevation of intraocular pressure (IOP) is a major risk factor associated with primary open angle glaucoma (POAG), a common form of progressive optic neuropathy that can lead to debilitating loss of vision. Recent studies have identified the role of
Joanna J Bartnicka et al.
Metallomics : integrated biometal science, 12(10), 1508-1520 (2020-09-23)
Copper imbalance is implicated in many diseases, including cancer. Copper in blood is mainly transported by carrier proteins but a small fraction is bound to low molecular weight species, possibly amino acids. Their roles in cellular copper delivery are unknown.
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