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A2153

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, ≥96% (agarose gel electrophoresis)

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Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

biological source

bovine

Quality Level

Assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

~66 kDa

purified by

cold ethanol fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

impurities

≤6.0% Loss on Drying

loss

≤6%

pH

6.5-7.5 (1% in 0.15 M sodium chloride)
7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

foreign activity

BT Virus, none detected
VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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This Item
A7511A7906A7638
Bovine Serum Albumin lyophilized powder, ≥96% (agarose gel electrophoresis)

Sigma-Aldrich

A2153

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, essentially fatty acid free, ≥97% (agarose gel electrophoresis), 1X Crystallized

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A7511

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, pH 7, ≥98%

Sigma-Aldrich

A7906

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

Sigma-Aldrich

A7638

Bovine Serum Albumin

assay

≥96% (agarose gel electrophoresis)

assay

≥97% (agarose gel electrophoresis)

assay

≥98%

assay

≥99% (agarose gel electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

purified by

cold ethanol fractionation

purified by

cold ethanol fractionation

purified by

heat shock fractionation

purified by

cold ethanol and heatshock fractionation

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

General description

Bovine Serum Albumin (BSA) is a globular, non-glycosylated α-helical plasma protein produced in the bovine liver. It consists of three domains (I, II, and III), each with two sub-domains (A and B), arranged in a heart-shaped structure.

Application

Bovine serum albumin has been used-

  • along with BTS (Beltsville thawing solution), for the extension of sperms during sperm motion analysis
  • as a component of modified Krebs-Ringer buffer (dmKRBT)
  • in gel shift analysis
  • as a blocking agent in immunocytochemistry, immunohistochemistry,(4) immunofluorescence protocol and sandwich enzyme-linked immunosorbent assay (ELISA)
  • as a component of phosphate buffer saline (PBS) solution to resuspend cells for analysis during flow cytometry
  • as a protein standard to quantify proteins in tissue samples via Bradford’s method
  • to measure the enzyme activity of 17β HSD enzyme

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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USP

USP

1076192

BSA for protein quantitation

Bovine Serum Albumin lyophilized powder, ≥98% (agarose gel electrophoresis)

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Bovine Serum Albumin lyophilized powder, essentially fatty acid free and essentially globulin free, ≥99% (agarose gel electrophoresis)

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Simple histochemical stain for acrosomes on sperm from several species.
Larson JL and Miller DJ
Molecular Reproduction and Development, 52(4), 445-449 (1999)
Bartolomeo Della Ventura et al.
PloS one, 12(2), e0171754-e0171754 (2017-02-10)
Biosensor-based detection provides a rapid and low-cost alternative to conventional analytical methods for revealing the presence of the contaminants in water as well as solid matrices. Although important to be detected, small analytes (few hundreds of Daltons) are an issue
Mateusz Majda et al.
Developmental cell, 43(3), 290-304 (2017-11-08)
The epidermis of aerial plant organs is thought to be limiting for growth, because it acts as a continuous load-bearing layer, resisting tension. Leaf epidermis contains jigsaw puzzle piece-shaped pavement cells whose shape has been proposed to be a result
Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry.
Guthrie HD and Welch GR
Journal of Animal Science, 84(8), 2089-2100 (2006)
Maleeha F Ahmad et al.
Arteriosclerosis, thrombosis, and vascular biology, 39(2), 188-199 (2018-12-21)
Objective- Perivascular adipose tissue (PVAT) contains an independent adrenergic system that can take up, metabolize, release, and potentially synthesize the vasoactive catecholamine norepinephrine. Norepinephrine has been detected in PVAT, but the mechanism of its protection within this tissue is unknown.

Related Content

Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.

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