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A3157

Sigma-Aldrich

Alcian Blue 8GX

certified by the Biological Stain Commission

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Synonym(s):
Alcian Blue, Ingrain Blue 1
Empirical Formula (Hill Notation):
C56H68Cl4CuN16S4
CAS Number:
Molecular Weight:
1298.86
Colour Index Number:
74240
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, ≥50%

solubility

water: 1 mg/mL, blue

εmax

195-400 at 617-630 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C

InChI

1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4

InChI key

CKLBXIYTBHXJEH-UHFFFAOYSA-J

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This Item
B538819821805500
Alcian Blue 8GX certified by the Biological Stain Commission

Sigma-Aldrich

A3157

Alcian Blue 8GX

Patent Blue VF Dye content 50 %

Sigma-Aldrich

198218

Patent Blue VF

Alcian Blue 8GX for microscopy (Bact., Bot., Hist.)

Sigma-Aldrich

05500

Alcian Blue 8GX

form

powder

form

powder

form

powder

form

powder

composition

Dye content, ≥50%

composition

Dye content, ≥60%

composition

Dye content, 50%

composition

-

solubility

water: 1 mg/mL, blue

solubility

water: 1 mg/mL, blue to very deep blue

solubility

H2O: 1 mg/mL

solubility

methanol: water (1:1): 0.01 g/10 mL, blue to very deep blue

εmax

195-400 at 617-630 nm

εmax

-

εmax

-

εmax

≥60 at 605-625 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

Application

Alcian Blue 8GX has been used for the staining of sulfated proteoglycans in murine MPC (mesenchymal progenitor cell) nodule. It has been used for the staining of sulfated proteoglycans in bovine articular chondrocytes.

Biochem/physiol Actions

Alcian Blue 8GX is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby helping in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.

Suitability

Certified for use in staining in Mowry′s pH 2.5 method, in Scott′s pH 5.7 method, and in Kreyberg′s method for keratin and mucin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

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To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.

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