A3678

Ammonium persulfate

for molecular biology, suitable for electrophoresis, ≥98%

• Synonym(s): AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER
• Linear Formula: (NH₄)₂S₂O₈
• CAS Number: 7727-54-0
• Molecular Weight: 228.20
• EC Number: 231-786-5
• MDL number: MFCD00003390
• eCl@ss: 38030421
• PubChem Substance ID: 24890800
• NACRES: NB.22

Properties

• grade: for molecular biology
• Quality Level: 200
• vapor density: 7.9 (vs air)
• assay: ≥98%
• form: powder
• reaction suitability: reagent type: oxidant
• technique(s): electrophoresis: suitable
• anion traces: chloride (Cl^-): <10 ppm
• cation traces: Fe: <10 ppm; heavy metals (as Pb): <50 ppm
• foreign activity: DNAse, none detected; Protease, none detected; RNAse, none detected
• SMILES string: N.N.OS(=O)(=O)OOS(O)(=O)=O
• InChI: 1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)
• InChI key: ROOXNKNUYICQNP-UHFFFAOYSA-N

Description

General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Application

Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.

Catalyst for acrylamide gel polymerization.

Packaging

10, 25, 100 g in poly bottle

Analysis Note

Tested for use in acrylamide polymerization.

Safety Information

• Pictograms: GHS03,GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H272 - H302 - H315 - H317 - H319 - H334 - H335
• Precautionary Statements: P210 - P220 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 5.1B - Oxidizing hazardous materials
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves