A4378

Bovine Serum Albumin

lyophilized powder, 1× crystallized, ≥97% (agarose gel electrophoresis)

• Synonym(s): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• NACRES: NA.27

Properties

• biological source: bovine
• Assay: ≥97% (agarose gel electrophoresis)
• form: lyophilized powder
• quality: crystallized
• mol wt: ~66 kDa
• purified by: cold ethanol fractionation
• packaging: poly bottle of
• origin: USA origin
• technique(s): ELISA: suitable
• pH: 7
• solubility: water: soluble (50 mg/ml)
• UniProt accession no.: P02769
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

General description

Albumin is the most abundant plasma protein in humans. High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Serum albumin is a reliable prognostic indicator for liver disease. Oxidative damage of albumin is associated with advanced liver disease.

Application

Bovine Serum Albumin has been:

• as an additive in washout solution and fluorescent dye solution
• as a supplement in Waymouth medium and modified essential medium-α (MEMα )
• in plant extract for the protein precipitation test
• as a component in Ringer′s solution to rinse the retinal tissue

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method. Further purified by a proprietary crystallization method

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves