Merck
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A8412

Sigma-Aldrich

Bovine Serum Albumin solution

7.5% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

Synonym(s):
Albumin solution from bovine serum, BSA solution
CAS Number:
MDL number:
NACRES:
NA.75

biological source

bovine

Quality Level

description

low endotoxin

sterility

sterile-filtered

product line

BioXtra

Assay

7.0-8.0% protein basis (UV)

form

liquid

mol wt

66.4 kDa

purified by

cold ethanol and heatshock fractionation

origin

USA origin

concentration

7.5% in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

≤13.0 EU/mL Endotoxin level

pH

6.5-7.5

foreign activity

Mycoplasma test (Negative)
cell growth >50%

shipped in

ambient

storage temp.

2-8°C

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This Item
A7979A1595A1662
sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

aseptically filled

assay

7.0-8.0% protein basis (UV)

assay

33-37% total protein basis (UV)

assay

90-120 mg/mL protein basis (biuret)

assay

28-32% protein basis (biuret)

form

liquid

form

liquid

form

solution

form

solution

mol wt

66.4 kDa

mol wt

-

mol wt

-

mol wt

-

purified by

cold ethanol and heatshock fractionation

purified by

-

purified by

heat shock fractionation

purified by

heat shock fractionation

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

Qualified for use in cell culture applications.

Biochem/physiol Actions

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media (SFM). It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Albumin in Cell Culture

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

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