B6917

Bovine Serum Albumin

lyophilized powder, suitable for (for molecular biology), Non-acetylated

• Synonym(s): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• NACRES: NA.27

Properties

• biological source: bovine
• grade: for molecular biology
• Assay: ≥98% (agarose gel electrophoresis)
• form: lyophilized powder
• quality: deacetylated
• mol wt: ~66 kDa
• purified by: heat shock fractionation
• packaging: glass bottle of
• origin: USA origin
• technique(s): electrophoresis: suitable-98% using Agarose
• impurities: enzymatic impurity, none detected
• pH: 7
• solubility: water: soluble (50 mg/ml)
• suitability: suitable for (for molecular biology)
• UniProt accession no.: P02769
• foreign activity: Alkaline Phosphatase free ; Alkaline phosphatase and peroxidase, none detected; DNase free ; DNase, RNase, and protease, none detected; Peroxidase free ; Protease free ; RNase free
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

Application

Select the right albumin for your application with the Albumin Selection Guide

BSA has various uses in molecular biology, notably as an inert protein component to function as a stabilizer for proteins and enzymes of principal activity in solution. In such applications, BSA thus works, for example, to reduce risk of adhesion of the enzyme or other protein of interest to the reaction tube surface/wall or to the pipette surface. Thus during reactions, molecular biology grade BSA allows the enzyme or protein of interest to function properly, with minimal risk to the integrity of the protein or enzyme of interest.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

This BSA product for molecular biology is tested for the following properties:
* Alkaline Phosphatase-free
* DNase-free / Exonuclease-free
* Nickase-free / Endonuclease-free
* Peroxidase-free
* Protease-free
* RNase-free

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves