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Merck

B9146

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

≥98%, powder, suitable for identification of lac+ bacterial colonies

Synonym(s):

5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

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RM 297.00
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RM 1,152.00
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RM 4,560.00

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About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
7240-90-6
Molecular Weight:
408.63
Beilstein:
1402009
EC Number:
230-640-8
MDL number:
MFCD00005666
UNSPSC Code:
12352204
PubChem Substance ID:
57653968
NACRES:
NA.83

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Product Name

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%

Quality Level

200

Assay

≥98%

form

powder

solubility

DMF: methanol (1:1): soluble 50 mg/mL

suitability

suitable for identification of lac+ bacterial colonies

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

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This Item
B4252B6024V900468
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside ≥98%

Sigma-Aldrich

B9146

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside ≥98%, powder

Sigma-Aldrich

B4252

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside tablet

Sigma-Aldrich

B6024

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside Vetec™, reagent grade, ≥98%

Sigma-Aldrich

V900468

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

assay

≥98%

assay

≥98%

assay

-

assay

≥98%

solubility

DMF: methanol (1:1): soluble 50 mg/mL

solubility

DMF: methanol (1:1): 50 mg/mL

solubility

-

solubility

DMF: methanol (1:1): 50 mg/mL, clear, colorless to light yellow

suitability

suitable for identification of lac+ bacterial colonies

suitability

-

suitability

suitable for identification of lac+ bacterial colonies

suitability

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

form

powder

form

powder

form

tablet

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used to confirm transduction of Caki-2/LacZ clones.[1] It has also been used in blue-white selection of transformants.[2]
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Preparation Note

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.

related product

Product No.
Description
Pricing

94433

β-Galactosidase stain

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000503315
0000503315
0000496949
0000496949
0000483117

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