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D3812

Sigma-Aldrich

DirectLoad 50 bp DNA Step Ladder

ready-to-use marker for DNA electrophoresis

NACRES:
NA.25

Quality Level

form

(liquid)

usage

sufficient for 100 loads

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s DirectLoad 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

DirectLoad 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay.
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Components

Sigma′s DirectLoad 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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