D5030

Dulbecco′s Modified Eagle′s Medium

Name: Dulbecco′s Modified Eagle′s Medium
Brand: SIGMA

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

DME, DMEM

Slide 1 of 1

Sign Into View Organizational & Contract Pricing

About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

Key Documents

View All Documentation

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: no
NaHCO₃: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item	D5921	D5523	D5648
D5030 Dulbecco′s Modified Eagle′s Medium	D5921 Dulbecco′s Modified Eagle′s Medium - low glucose	D5523 Dulbecco′s Modified Eagle′s Medium - low glucose	D5648 Dulbecco′s Modified Eagle′s Medium - high glucose
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form liquid	form powder	form powder
components phenol red: no NaHCO₃: no L-glutamine: no HEPES: no glucose: no sodium pyruvate: no	components phenol red: no sodium pyruvate: no glucose: low HEPES: no L-glutamine: no NaHCO₃: yes	components L-glutamine: yes NaHCO₃: no sodium pyruvate: yes phenol red: yes HEPES: no glucose: low	components phenol red: yes L-glutamine: yes NaHCO₃: no HEPES: no glucose: high sodium pyruvate: no
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
Quality Level 500	Quality Level 400	Quality Level 500	Quality Level -

General description

Dulbecco′s Modified Eagle′s Medium (DME) is a modification of Basal Medium Eagle (BME) that has been formulated with a 4-fold higher concentration of amino acids and vitamins. It includes additional supplementary components. The DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. Modifying the medium with 4,500 mg/L glucose is optimal for culturing certain cell types.

The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.

Application

Dulbecco′s Modified Eagle′s Medium has been used:

to culture the isolated flexor digitorum brevis (FDB) fibers to measure exogenous fatty acid (FA) utilization as part of oxygen consumption rate (OCR) measurements
to culture the isolated tibialis anterior (TA) muscle fibers for lactate measurements
to culture the human bone marrow mesenchymal stem cells for osteogenic differentiation to prepare a low-glucose medium to culture human hepatocellular carcinoma HepG2 cells

Quantity

Formulated to contain 8.3 grams of powder per liter of medium.

Reconstitution

Supplement with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Pictograms

GHS07

Signal Word

Warning

Hazard Statements

H319

Precautionary Statements

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service