D8662

Dulbecco′s Phosphate Buffered Saline

With MgCl₂ and CaCl₂, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): DPBS
• MDL number: MFCD00131855
• eCl@ss: 32129211
• NACRES: NA.75

Properties

• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: sodium pyruvate: no; glucose: no (Dextro)
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.

Application

D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.

Dulbecco′s Phosphate Buffered Saline has been used:

• as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
• to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
• to wash cells for protein isolation

Dulbecco′s Phosphate Buffered Saline has been used:

• as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
• to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
• to wash cells for protein isolation

Packaging

100, 500, 500, 500 mL

1, 1 L

Biochem/physiol Actions

Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:

• provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
• serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
• when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
• provides a buffering system to maintain the medium within the physiological pH range

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable