Sigma′s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Sigma′s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Contains 8 fragments, 125–23,130 bp
For optimal resolution, the recommended agarose gel concentration is 0.75%.
Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.