I8896

IGEPAL^® CA-630

for molecular biology

• Synonym(s): Octylphenoxy poly(ethyleneoxy)ethanol, branched
• Linear Formula: (C₂H₄O)_nC₁₄H₂₂O
• CAS Number: 9002-93-1
• NACRES: NA.31

Properties

• grade: for molecular biology
• Quality Level: 200
• description: non-ionic
• form: liquid
• technique(s): IR spectroscopy: suitable
• CMC: 0.08 mM (20-25°C)
• HLB: 13
• foreign activity: DNase and RNase, none detected
• InChI: 1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3
• InChI key: JYCQQPHGFMYQCF-UHFFFAOYSA-N

Description

General description

IGEPAL^® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL^® CA-630 has been used

• as neutral detergent in homogenizing buffer during western blotting of mouse muscle homogenates
• In X-gal staining for studying the lacZ expression in E8.5-E10.5 embryos
• In Cell-Lysis (CL) buffer for preparing RT-qPCR-ready cell lysates

Other Notes

Nonionic, non-denaturing detergent

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Caution

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Legal Information

IGEPAL is a registered trademark of Solvay

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302 - H315 - H318 - H410
• Precautionary Statements: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 483.8 °F
• Flash Point(C): 251 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves