L3037

Escort^™ III Transfection Reagent

Lipid reagent for transfecting sensitive and primary cells

• UNSPSC Code: 41106502
• NACRES: NA.25

Properties

• grade: Molecular Biology
• Quality Level: 200
• form: liquid (aqueous solution)
• usage: 1 mL sufficient for 250-1000 transfections
• concentration: 1 mg/mL
• technique(s): transfection: suitable
• storage temp.: 2-8°C

Description

General description

Escort^™ III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 μl Escort^™ III and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort^™ III:

A549
C2C12 myotubes
Cardiomyocytes (rat)
COS-7
Fibroblasts (rat)
Germ cells (male rat)
Hepatocytes (rat and hamster)
HepG2
HeLa
Jurkat
Keratinocytes (human)
Myoblasts (mouse and quail)
Myocytes (mouse)
NIH3T3
PC-12
Retinal Neurons (rat)
Tracheobronchial cells (sheep)

Features and Benefits

• Suitable for stable and transient transfection
• Optimized for a wide variety of primary cells
• Low toxicity
• Compatible with both serum and serum-free transfection protocols
• Ideal for PC-12 cells

Components

Escort^™ III formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort^™ III is confidential.

Caution

Do not freeze.

Principle

A stable complex is formed when Escort^™ III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves