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M5524

Sigma-Aldrich

Murashige and Skoog Basal Salt Mixture (MS)

powder, suitable for plant cell culture

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Synonym(s):
MS Basal Salts, MS0 Basal Salts, MSO Basal Salts
NACRES:
NA.72

form

powder

Quality Level

100

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

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M0654M0529M6899
Murashige and Skoog Basal Salt Mixture (MS) powder, suitable for plant cell culture

Sigma-Aldrich

M5524

Murashige and Skoog Basal Salt Mixture (MS)

Sigma-Aldrich

Sigma-Aldrich

M0654

Murashige and Skoog Basal Salt Macronutrient Solution

Sigma-Aldrich

Sigma-Aldrich

M0529

Murashige and Skoog Basal Salt Micronutrient Solution

Sigma-Aldrich

Sigma-Aldrich

M6899

Murashige and Skoog Basal Salts with minimal organics

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

200

General description

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and Kinetin (cytokinin/cell division promoter).

Formula variant

With the macro- and micronutrients as described by Murashige and Skoog (1962).

Media Formulation

Quantity

Formulated to contain 4.3 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M5524) contains the macronutrients and micronutrients of the original classic formulation. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Other Notes

Storage
Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.

Store dry medium in a desiccator at 0-5 °C.

Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used.

Media Preparation

related product

Product No.
Description
Pricing

Pictograms

Flame over circleExclamation mark
GHS03,GHS07

Signal Word

Warning

Hazard Statements

H272 - H319

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Articles

Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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