Merck
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M5755

Sigma-Aldrich

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

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Synonym(s):
MESA Buffer

grade

for molecular biology

product line

BioReagent

form

powder

pH

6.8-7.2(reconstituted per directions)

solubility

H2O: soluble, faintly yellow

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

room temp

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T8280PPB00867996
MOPS-EDTA-Sodium Acetate (MESA) powder for RNA electrophoresis buffers

Sigma-Aldrich

M5755

MOPS-EDTA-Sodium Acetate (MESA)

Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Sigma-Aldrich

T8280

Tris Acetate-EDTA buffer

Tris Acetate-EDTA buffer pH 8.3, pHast Pack™, powder

Sigma-Aldrich

PPB008

Tris Acetate-EDTA buffer

Sigma-Aldrich

Sigma-Aldrich

67996

Tris Acetate-EDTA buffer Concentrate

grade

for molecular biology

grade

for molecular biology

grade

-

grade

for molecular biology

product line

BioReagent

product line

BioReagent

product line

pHast Pack™

product line

-

form

powder

form

powder blend

form

powder

form

solution

pH

6.8-7.2(reconstituted per directions)

pH

8.1-8.5

pH

8.3

pH

8.3±0.2 (25 °C)

solubility

H2O: soluble, faintly yellow

solubility

-

solubility

-

solubility

-

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

foreign activity

-

foreign activity

DNAse, none detected, Nickase, none detected, Protease, none detected, RNAse, none detected

foreign activity

-

General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Components

1x MESA buffer contains:
  • 40 mM MOPS
  • 10 mM sodium acetate
  • 1 mM EDTA (pH 8.3)

Reconstitution

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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