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M7167

Sigma-Aldrich

M2 medium

With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

Synonym(s):

Cell Culture M2, Cell Culture Medium, M2 Medium Solution

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RM 246.00
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RM 418.00

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50 ML
RM 246.00
100 ML
RM 418.00

About This Item

MDL number:
MFCD00283761
UNSPSC Code:
12352207
NACRES:
NA.75

RM 246.00

Check Cart for Availability
Request a Bulk Order
Notify Me

Get notified when this item is ready to ship via email.

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

components

HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L

shipped in

ambient

storage temp.

2-8°C

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Application

Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.

Quality

Presence of particulates in solution will not affect product′s performance.

Reconstitution

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Analysis Note

This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000360797
0000360795
0000360859
0000349109
0000349109

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Jane C Fenelon et al.
Biology of reproduction, 97(1), 119-132 (2017-06-24)
Embryonic diapause is a common reproductive strategy amongst mammals, requiring an intimate cross-talk between the endometrium and the blastocyst. To date, the precise molecular signals responsible are unknown in the mouse or any other mammal. Previous studies in the mink
Sarah E M Stephenson et al.
Scientific reports, 8(1), 7528-7528 (2018-05-16)
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Hongzheng Sun et al.
Biology of reproduction, 100(3), 601-617 (2018-09-29)
Methionine adenosyltransferase II (MAT2A) is essential to the synthesis of S-adenosylmethionine, a major methyl donor, from L-methionine and ATP. Upon fertilization, zygotic genome activation (ZGA) marks the period that transforms the genome from transcriptional quiescence to robust transcriptional activity. During
Maryse Lessard et al.
Scientific reports, 9(1), 13829-13829 (2019-09-27)
The paternal environment is thought to influence sperm quality and future progeny may also be impacted. We hypothesized that prenatal exposure to environmentally-relevant contaminants impairs male reproduction, altering embryo gene expression over multiple generations. Folic acid (FA) can improve sperm
Mariana Schroeder et al.
Nature communications, 9(1), 1596-1596 (2018-04-25)
Anorexia nervosa (AN) is a devastating eating disorder characterized by self-starvation that mainly affects women. Its etiology is unknown, which impedes successful treatment options leading to a limited chance of full recovery. Here, we show that gestation is a vulnerable
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