Iron is a mineral that plays an essential role in many biological processes, including DNA synthesis, electron transport and oxygen transport. Iron is a transition element that can form a range of oxidation states, the most common being iron II (Fe2+ or ferrous iron) and iron III (Fe3+ or ferric iron). Iron-containing proteins participate in many reactions, often utilizing transitory changes in the oxidation state of iron to carry out chemical reactions.
Iron assay kit has been used to determine the concentration of Fe2+ in wild type (WT) and Δ ferric uptake regulator (Fur) strains of A. baumannii.
Suitable for the measurement of iron concentration in various samples
In this assay, iron is released by the addition of an acidic buffer. Samples may be tested directly to measure Fe2+ or reduced to measure total iron (Fe2+ and Fe3+). Released iron is reacted with a chromagen resulting in a colorimetric (593 nm) product, proportional to the iron present. The Iron Assay Kit provides a simple convenient means of measuring iron in a variety of biological samples.
Kit is compatible with serum and tissue samples but not plasma samples.