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MAK039

Sigma-Aldrich

Acetyl-Coenzyme A Assay Kit

sufficient for 100 fluorometric tests

Synonym(s):

Acetyl-CoA Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

cancer; neurological disorders

storage temp.

−20°C

General description

Acetyl-CoA is an essential cofactor and carrier of acyl groups in enzymatic acetyl transfer reactions. It is formed either by the oxidative decarboxylation of pyruvate in mitochondria, by the oxidation of long-chain fatty acids, or by the oxidative degradation of certain amino acids. Acetyl-CoA is the starting compound for the citric acid cycle (Kreb′s cycle). It is also a key precursor in lipid biosynthesis, and the source of all fatty acid carbons. Acetyl-CoA positively regulates the activity of pyruvate carboxylase. It is a precursor of the neurotransmitter acetylcholine. Histone acetylases (HAT) use Acetyl-CoA as the donor for the acetyl group used in the post-translational acetylation reactions of histone and non-histone proteins.

Acetyl-Coenzyme A acts as an indicator of fat, sugar, and protein levels and shows up on the nutritional status. Thus, starvation greatly reduces the level of acetyl-CoA, which stimulates the process of autophagy. In lowered glucose condition, acetyl-CoA participates in the production of ATP. Increased acetyl-CoA levels are observed in prostate cancer due to elevated fatty acid utilization and thus provides an additional energy source for the tumor cell growth. Nutrition deprivation, changes the levels of certain metabolites, including acetyl-CoA, which stimulates epigenetic modifications that would affect the central nervous system.

Application

Acetyl-Coenzyme A Assay Kit has been used to quantify the level of acetyl-CoA in astrocytes exposed to recombinant HIV protein Tat and Cocaine. It has also been used to study the mitochondrial metabolism by evaluating the levels of acetyl-CoA using hypoxia induced stress in primary human ventricular cardiomyocytes.

Suitability

Suitable for the measurement of Acetyl CoA in a variety of biological samples

Principle

Acetyl-CoA concentration is determined by a coupled enzyme assay, which results in a fluorometric (λex = 535/λem = 587 nm) product, proportional to the Acetyl-CoA present. Typical sensitivities of detection for this kit are 10-1000 pmole of Acetyl CoA. This kit is a highly sensitive assay for determining Acetyl-CoA level in a variety of biological samples.

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Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup


Certificates of Analysis (COA)

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Regulation of autophagy by cytosolic acetyl-coenzyme A
Marino G, et al.
Molecular Cell, 53(5), 710-725 (2014)
Fatty acid oxidation is a dominant bioenergetic pathway in prostate cancer
Liu Y.
Prostate Cancer and Prostatic Diseases, 9(3), 230-230 (2006)
Ziqiang Wang et al.
Cellular and molecular life sciences : CMLS, 76(15), 3005-3018 (2019-04-22)
The accumulation of intracellular β-amyloid peptide (Aβ) is important pathological characteristic of Alzheimer's disease (AD). However, the exact underlying molecular mechanism remains to be elucidated. Here, we reported that Nuclear Paraspeckle Assembly Transcript 1 (NEAT1), a long n on-coding RNA
Nanocurcumin prevents hypoxia induced stress in primary human ventricular cardiomyocytes by maintaining mitochondrial homeostasis.
Nehra S, et al.
PLoS ONE, 10(9), e0139121-e0139121 (2015)
Maria I Dauden et al.
Science advances, 5(7), eaaw2326-eaaw2326 (2019-07-17)
The highly conserved Elongator complex modifies transfer RNAs (tRNAs) in their wobble base position, thereby regulating protein synthesis and ensuring proteome stability. The precise mechanisms of tRNA recognition and its modification reaction remain elusive. Here, we show cryo-electron microscopy structures

Articles

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

Fatty acid synthesis supports cancer cell proliferation, essential for membrane generation, protein modification, and bioenergetics.

Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.

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