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MAK094

Sigma-Aldrich

Protein Carbonyl Content Assay Kit

sufficient for 100 colorimetric tests

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NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cardiovascular diseases; neurological disorders; cancer; gastrointestinal diseases

storage temp.

2-8°C

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Protein Carbonyl Content Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK094

Protein Carbonyl Content Assay Kit

Antioxidant Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK334

Antioxidant Assay Kit

Aconitase Activity Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK051

Aconitase Activity Assay Kit

Lipid Peroxidation (MDA) Assay Kit sufficient for 100 colorimetric or fluorometric tests

Sigma-Aldrich

MAK085

Lipid Peroxidation (MDA) Assay Kit

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric, fluorometric

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

relevant disease(s)

cardiovascular diseases; neurological disorders; cancer; gastrointestinal diseases

relevant disease(s)

neurological disorders

relevant disease(s)

neurological disorders; cardiovascular diseases; cancer

relevant disease(s)

endocrinological disorders, diabetes; neurological disorders; cancer; cardiovascular diseases

General description

Oxidative stress results when the effectiveness of antioxidant defenses is insufficient to deal with the production of reactive oxygen species (ROS). ROS can induce damage to DNA, lipids, and proteins. The oxidation of proteins results in the production of stable carbonyl groups, which can be used as a measure of oxidative injury.

Application

Protein carbonyl content assay kit has been used to determine protein carbonyl concentration.

Suitability

Suitable for quantifying carbonyls in protein samples

Principle

The Protein Carbonyl Content Assay Kit provides a simple and direct procedure for measuring carbonyl content in a variety of biological samples. Carbonyl content is determined by the derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH) leading to the formation of stable dinitrophenyl (DNP) hydrazone adducts, which can be detected spectrophotometrically at 375 nm, proportional to the carbonyls present.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

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