Merck
All Photos(1)

Documents

MAK133

Sigma-Aldrich

ADP Assay Kit

sufficient for 100 assays (bioluminescent)

All Photos(1)
NACRES:
NA.84

usage

sufficient for 100 assays (bioluminescent)

detection method

chemiluminescent

relevant disease(s)

hematological disorder; cancer

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MAK001MAK190MAK059
ADP Assay Kit sufficient for 100 assays (bioluminescent)

Sigma-Aldrich

MAK133

ADP Assay Kit

Alanine Assay Kit sufficient for 100 colorimetric or fluorometric tests

Sigma-Aldrich

MAK001

Alanine Assay Kit

ATP Assay Kit Sufficient for 100 Colorimetric or Fluorometric tests

Sigma-Aldrich

MAK190

ATP Assay Kit

Formate Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK059

Formate Assay Kit

detection method

chemiluminescent

detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

detection method

colorimetric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Adenosine diphosphate (ADP) is a nucleoside that plays a critical role in energy transfer reactions. ADP is produced from adenosine triphosphate via the action of ATPases. ADP also plays a critical role in platelet function. ADP, stored in plate-dense granules, is released upon platelet activation where it acts on purinergic receptors to mediate intracellular signaling and platelet aggregation.

The ADP Assay kit provides a simple and direct procedure for measuring ADP levels in cells and other biological samples. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presences of luciferse, ATP immediately reacts with the Substrate (D-luciferin) to produce light. The light intensity is a direct measure of the intracellular ATP concentration and is stable over several minutes.

In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample.

Suitability

Suitable for the detection of ADP in cells, tissue and other biological samples.

Principle

The ADP Assay kit provides a simple and direct procedure for measuring ADP levels in cells and other biological samples. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presences of luciferse, ATP immediately reacts with the substrate (D-luciferin) to produce light. The light intensity is a direct measure of the intracellular ATP concentration and is stable over several minutes.
Luciferase ATP + D-Luciferin + O2 → oxyluciferin + AMP + PPi + CO2 + light
In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317 - H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 3

1 of 3

Adenosine 5′-diphosphate sodium salt bacterial, ≥95% (HPLC)

Sigma-Aldrich

A2754

Adenosine 5′-diphosphate sodium salt

NAD/NADH Quantitation Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK037

NAD/NADH Quantitation Kit

Sigma-Aldrich

Sigma-Aldrich

M3003

Myokinase from rabbit muscle

Sepinoud Firouzmand et al.
Neurourology and urodynamics, 39(3), 926-934 (2020-02-13)
To characterize purinergic signaling in overactive bladder (OAB). Mucosal biopsies were taken by flexible cystoscopy from patients with storage symptoms referred to Urology Departments of collaborating hospitals. Immunohistochemistry (n = 12) and Western blot analysis (n = 28) were used to establish the qualitative
Aggregation of blood platelets by adenosine diphosphate and its reversal.
Born G V R
Nature, 194(4832), 927-929 (1962)
The membrane ATPase of Escherichia coli: I. Ion dependence and ATP-ADP exchange reaction.
Roisin M P and Kepes A
Biochimica et Biophysica Acta - Bioenergetics, 275(3), 333-346 (1972)
Hongshan Ge et al.
Molecular and cellular endocrinology, 443, 128-137 (2017-01-17)
To explore the roles of mitochondrial Uncoupling Protein 2 (UCP2) in cumulus cells (CCs), human CCs were cultured in vitro, and the UCP2 was inhibited by treatment with Genipin, a special UCP inhibitor, or by RNA interference targeting UCP2. No significant
Sofia Doello et al.
Current biology : CB, 31(8), 1606-1615 (2021-02-12)
The ability to resume growth after a dormant period is an important strategy for the survival and spreading of bacterial populations. Energy homeostasis is critical in the transition into and out of a quiescent state. Synechocystis sp. PCC 6803, a
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service