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P1598

Sigma-Aldrich

PCR 20 bp Low Ladder

for electrophoresis of PCR fragments

NACRES:
NA.25

grade

for molecular biology

Quality Level

form

liquid

usage

80 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

2-8°C

General description

Sigma′s PCR 20 bp Low Ladder contains 50 dsDNA fragments from 50 – 1,000 bases, evenly spaced in 20 bp repeats. Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

PCR 20 bp Low Ladder has been used as a marker for DNA gel electrophoresis.

Components

Sigma′s PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 50 bands, 20–1,000 bp in exact 20 bp repeats.
For optimal resolution, the recommended agarose gel concentration is 2.5%.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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