Sigma′s PCR 20 bp Low Ladder contains 50 dsDNA fragments from 50 – 1,000 bases, evenly spaced in 20 bp repeats. Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
PCR 20 bp Low Ladder has been used as a marker for DNA gel electrophoresis.
Sigma′s PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Contains 50 bands, 20–1,000 bp in exact 20 bp repeats.
For optimal resolution, the recommended agarose gel concentration is 2.5%.