QPBCA

QuantiPro^™ BCA Assay Kit

for 0.5-30 μg/ml protein

• Synonym(s): BCA Assay, Protein determination by BCA, bicinchoninic acid reagent
• NACRES: NA.84

Properties

• Quality Level: 200
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

In addition to protein determination and quantification in solution, the QuantiPro^™ BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.

The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.

Features and Benefits

• Accurate across a broad range of protein concentrations
• High sensitivity; linear response form 0.5 to 30 μg/ml of protein
• Stable color complex
• Reduced susceptibility to detergents

Principle

Based on the alkaline reduction of Cu²⁺ to Cu¹⁺ by proteins, and the formation of a bicinchoninic acid:Cu¹⁺ complex having an absorbance maximum at 562 nm.

Storage and Stability

Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.

Legal Information

QuantiPro is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

Kit Components Also Available Separately

• P0914 Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1

Safety Information

• Pictograms: GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H290 - H315 - H318 - H410
• Precautionary Statements: P234 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2
• Storage Class Code: 8A - Combustible, corrosive hazardous materials
• WGK: WGK 3