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S3401

Sigma-Aldrich

Sample Buffer, Laemmli 2× Concentrate

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Synonym(s):
SDS Sample Buffer
NACRES:
NA.25

packaging

vial of 6 mL

Quality Level

100
200

storage temp.

−20°C

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SSC Buffer 20× Concentrate for Northern and Southern blotting, solution

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SSC Buffer 20× Concentrate

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S2015

SSPE Buffer 20× Concentrate

Gel Loading Buffer for NA electrophoresis

G2526

Gel Loading Buffer

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

packaging

vial of 6 mL

packaging

-

packaging

-

packaging

-

Application

Sample Buffer, Laemmli 2× concentrate has been used as a sample buffer for denaturing and loading of protein samples in SDS-PAGE.

Biochem/physiol Actions

Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well.

Other Notes

Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8.

Quantity

Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.

related product

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

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