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T4038

Sigma-Aldrich

Tris Acetate-EDTA buffer

DNase and RNase, none detected, BioReagent, suitable for electrophoresis, 10× concentrate

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Synonym(s):
TAE buffer
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

product line

BioReagent

form

powder blend

concentration

8.5-10.5 ×

impurities

DNase and RNase, none detected

pH

8.1-8.5

solubility

water: 64.2 g/L, clear, colorless

suitability

suitable for electrophoresis
suitable for gel electrophoresis (after dilution to working concentration)

application(s)

diagnostic assay manufacturing

SMILES string

CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

HGEVZDLYZYVYHD-UHFFFAOYSA-N

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Application

Ready for use in gel electrophoresis after dilution to working concentrations.
TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

Packaged in pouches

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Reconstitution

Produces a 10× concentrate (0.4 M Tris-acetate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water. A suitable container must be supplied.

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

STOT RE 2 Inhalation

Target Organs

Respiratory Tract

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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