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T5912

Sigma-Aldrich

Tris Buffered Saline

10 ×, solution

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Synonym(s):
TBS
MDL number:
NACRES:
NA.25

sterility

0.2 μm filtered

Quality Level

form

solution

concentration

10 ×

storage temp.

2-8°C

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This Item
T9039T6789T5030
Sigma-Aldrich

Sigma-Aldrich

T5912

Tris Buffered Saline

Tris Buffered Saline tablet

Sigma-Aldrich

T5030

Tris Buffered Saline

form

solution

form

powder

form

powder

form

tablet

concentration

10 ×

concentration

-

concentration

-

concentration

-

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Tris Buffered Saline has been used as washing buffer for electrodes. It was used to wash microtiter plates for western blot experiments and screening methods.

Other Notes

Washing buffer for alkaline phosphatase or peroxidase conjugates in Western blotting. Working solution will contain 20 mM Tris, pH approx. 7.4, and 0.9% NaCl

Suitability

Suitable for use as a washing buffer for alkaline phosphatase or peroxidase conjugates in dot blot assay.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Larry H Stanker et al.
Journal of immunological methods, 336(1), 1-8 (2008-05-03)
Botulinum neurotoxins (BoNT), produced by the anaerobic bacterium Clostridium botulinum, cause severe neuroparalytic disease and are considered the most toxic biological agents known. While botulism is rare in the U.S. it often is fatal if not treated quickly, and recovery
Rapid prototyping of multifunctional microfluidic cartridges for electrochemical biosensing platforms
Kin J
Sensors and Actuators B, Chemical, 202, 60-66 (2014)
Lucia Trisolini et al.
Biomolecules, 11(1) (2021-01-06)
ADP/ATP carriers (AACs) are mitochondrial transport proteins playing a strategic role in maintaining the respiratory chain activity, fueling the cell with ATP, and also regulating mitochondrial apoptosis. To understand if AACs might represent a new molecular target for cancer treatment
Edward Greenfield et al.
eLife, 3 (2014-12-11)
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered Report describes the proposed replication plan
Brad Evans et al.
eLife, 4, e07420-e07420 (2015-08-01)
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010

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Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

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