Merck
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T7777

Sigma-Aldrich

Tris-Glycine-SDS Buffer 10× Concentrate

Synonym(s):
TRIS glycine SDS buffer solution
eCl@ss:
32129211
NACRES:
NA.25

sterility

0.2 μm filtered

Quality Level

form

liquid

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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General description

Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Application

Tris-glycine-SDS buffer 10× concentrate has been used as a running buffer in sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE).[1][2] It is also used to block membranes with 5% non-fat dry milk for western blotting.[3] TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Reconstitution

Dilution of the 10× TGS buffer produces a 1× running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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