TP0100

Total Protein Kit, Micro

• Synonym(s): Bradford

Properties

• description: wavelength 595 nm; total test time 2 min; working reagent stability 18-26°C for 2 weeks; dynamic range 50 mg/dL 0.5 g/L
• Quality Level: 100
• parameter: 18-26 °C temperature; 50 μL sample volume
• storage temp.: 2-8°C

Description

General description

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.

Application

Total protein kit, micro has been used to determine:

• total protein in the bacterial homogenate by Bradford method
• total protein content by the Bradford microassay from the tissue extracts
• serum total protein
• soluble protein content by the Bradford method from leaves

Biochem/physiol Actions

Total protein kit, micro can be used to determine the concentration of proteins in solution.

Suitability

Suitable for protein determination for samples of up to 0.5 mg/ml.

Principle

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.

Testing Method

Colorimetric, Endpoint

Kit Components Also Available Separately

• Protein Standard 5 mL

Safety Information

• Pictograms: GHS02,GHS05,GHS07,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H226 - H290 - H302 + H312 + H332 - H314 - H317 - H370 - H411
• Precautionary Statements: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 132.8 °F
• Flash Point(C): 56 °C