565348-U

Ascentis^® RP-Amide HPLC Column

5 μm particle size, L × I.D. 25 cm × 21.2 mm

• eCl@ss: 32110501
• NACRES: SB.52

Properties

• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L60
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• extent of labeling: 19.5% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable
• L × I.D.: 25 cm × 21.2 mm
• surface coverage: 2.7 μmol/m²
• impurities: <5 ppm metals
• matrix: fully porous particle; silica gel high purity, spherical
• matrix active group: amide, alkyl phase
• particle size: 5 μm
• pore size: 100 Å
• surface area: 450 m²/g
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Features and Benefits

• Excellent retention and peak shape for polar compounds
• 100% aqueous compatibility
• Ultra low bleed, LC-MS compatible
• Unique selectivity

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC