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Determination of Hormones in Drinking Water by Solid Phase Extraction (SPE) and LC-MS/MS by EPA Method 539
A workflow utilizing an LC-MS/MS in combination with Ascentis® column and Supelco® chromatography consumables offers an efficient way to analyze hormones from drinking water by following EPA Method 539.
Genotypes, Phenotypes and Markers
A genotype is a list of mutant genes in an organism. In addition to mutations of the genome, other genetic elements such as prophage or plasmids can also be included.
Analysis of Pesticides in Turmeric Powder by LC/MS/MS and GC/MS/MS after Cleanup with Dual-Layer SPE Cartridge
Pesticide residue testing of turmeric and other (dried) spices is challenging due to their complex compositions and high matrix. An efficient SPE clean-up can be achieved by a new Dual-layer cartridge.
Electrospun Nanofibers for Drug Delivery Systems
Local delivery of bioactive molecules using an implantable device can decrease the amount of drug dose required as well as non-target site toxicities compared to oral or systemic drug administration.
GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol
GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA
Primary Neuron Culture and Analysis Facilitated by the CellASIC® ONIX Microfluidic Platform
This page describes a novel, optimized microfluidic platform for long-term culture of primary neurons to monitor dynamic cellular processes in real time.
Proline Derivatives and Analogs
Proline analogues are promising candidates for tuning the biological, pharmaceutical, or physicochemical properties of naturally occuring, as well as de novo designed, linear, and, cyclic peptides.
Conjugated Polymers for the Engineering of Device/Tissue Interface
Conjugated polymers offer charge transport between inorganic, electrically conducting metals and organic, proton-conducting biological systems.
Injectable Hydrogels for Cell Delivery and Tissue Regeneration
The use of hydrogel-based biomaterials for the delivery and recruitment of cells to promote tissue regeneration in the body is of growing interest. This article discussed the application of hydrogels in cell delivery and tissue regeneration.
Whole Genome Amplification for Single Cell Biology
Whole genome amplification (WGA) offers a means to overcome the above restrictions for single-cell genomic analyses. WGA has been described as a non-specific amplification technique that affords an amplified product completely representative of the initial starting material.
Whole Genome Amplification for Single Cell Biology
The ultimate biological unit lies within a single cell. Many biological disciplines have
taken aim to elucidate the causes of cellular differentiation at this level.
Synthesis and Biomedical Applications of Polyamino Acids
Polyamino acids are able to adopt ordered conformations, such as α-helices and β-sheets, through cooperative hydrogen bonding. These conformations impart polyamino acids with various unique properties and functions in biological environments.
Cell Migration Assay Guidance using Millicell® Hanging Cell Culture Inserts
How to set up cell migration assays using cell culture inserts
Ficoll® 400 for Gradient Centrifugation
Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.
Molecular Solar Thermal Energy Storage Systems (MOST) – Design, Synthesis, and Application
The substantial rise in global population has triggered a significant surge in the demand for energy worldwide, amplifying the need for renewable energy sources and improved energy storage. Molecular thermal energy storage (MOST) is a promising, highly controlled technique for
Synthesis, Properties, and Applications of Perovskite-Phase Metal Oxide Nanostructures
Synthesis, Properties, and Applications of Perovskite-Phase Metal Oxide Nanostructures
NF-κB and Inflammation
Chronic inflammation is an underlying factor in the development and progression of many of the chronic diseases of aging, such as arthritis, atherosclerosis, diabetes, and cancer.
PCR/qPCR Data Analysis
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
Poly(Glycerol Sebacate) in Tissue Engineering and Regenerative Medicine
The world of commercial biomaterials has stagnated over the past 30 years as few materials have successfully transitioned from the bench to clinical use. Synthetic aliphatic polyesters have continued to dominate the field of resorbable biomaterials due to their long
GenomePlex Whole Genome Amplification Kit Protocol (WGA1)
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
Barrier Formation and Permeability Assays using Millicell® Hanging Cell Culture Inserts
How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.
Gold Nanorod Synthesis
Plasmonic nanoparticles have unique optical properties that can be tailored to suit a variety of applications in the biotechnology1–8 and electronics9–16 industries.
The ELISpot Assay Enables Functional Analysis of Cellular Immunology
The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.
Protein- and Peptide- Polymer Conjugates by RAFT Polymerization
The modification of biomacromolecules, such as peptides and proteins, through the attachment of synthetic polymers has led to a new family of highly advanced biomaterials with enhanced properties.
Development of a Method for Analysis of Phthalate Esters in Vegetable Oils
Rapid, easy, sensitive method for the anaysis of PAEs, using LLE and direct immersion SPME, demonstrated good accuracy, repeatability, linearity and LOQ.
Immunohistochemistry: Current Applications in Skin Cancer
Immunohistochemistry (IHC) techniques and applications have greatly improved, dermatopathology is still largely based on H&E stained slides.This paper outlines ways in which IHC antibodies can be utilized for dermatopathology.
Chromatofocusing: Principles and Methods
This page covers the principles and methods of chromatofocusing, a chromatography technique that separates proteins according to differences in their isoelectric point (pI).
Overview of Luminex® Multiplex Assay Technology | MILLIPLEX® Multiplex Assays
Discover the benefits of MILLIPLEX® multiplex assays, based on Luminex® xMAP® multiplex assay technology, that provide consistent, high-quality results. See how these multiplex biomarker Luminex® assays are being used to advance research for immunology, cancer, veterinary medicine, metabolism/endocrinology, and more.