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Applications:Biologics and Biosimilars Characterization
Content Type:Protocol
Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
Released N-Linked Glycan Analysis Workflow of Adalimumab
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.
Protocol for Purification, Optional Reduction, and SEC-MS Analysis of a Monoclonal Antibody
A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.
Workflow for High-Throughput Glycoprofiling of Rituximab
Step-by-step SEC-MS protocol for rapid glycoprofiling of a monoclonal antibody (mAb), consisting of antibody purification, a mAb reduction procedure, a mass spectrometer calibration, and a system suitability test.
Workflow for Antibody Aggregate and Fragment Analysis
A complete SEC-UV workflow for quantifying monoclonal antibody fragments, monomers, and aggregates, including procedure for a system suitability test and heat stressing of antibodies.