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Applications:Biologics and Biosimilars Characterization
Content Type:Technical Article
Workflow for the Analysis of Polysorbate 80 in Erbitux® Formulation
A complete reversed phase HPLC-ELSD workflow, including sample preparation for the analysis of Polysorbate 80 in a monoclonal antibody formulation.
Difluoroacetic Acid as an Effective Mobile Phase Modifier for the LC-UV/MS Analysis of Proteins
The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.
Development of a Simple and Rapid Digestion Protocol for Proteomics Sample Preparation
In this study, we developed a rapid trypsin digest kit that, at elevated temperatures, yielded reliable, reproducible results in less than 2 hours on a wide variety of substrates for mass spectrometry.
Rapid Trypsin Digestion of Complex Protein Mixtures for Proteomics Analysis
Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation.
Strategies for Deglycosylating N-Linked Glycans
Explore various strategies for deglycosylating N-linked glycans involving PNGase F, PNGase A (Glycopeptidase A), and even native and sequential deglycosylation with endoglycosidases like Endoglycosidase H, Endoglycosidase F, and exoglycosidases.
Fast and high-resolution Analysis of Intact Therapeutic Monoclonal Antibody Trastuzumab using BIOshell™ A400 Protein C4 Column
Optimization of a Reversed-Phase Liquid Chromatographic (RP-LC) method for the intact analysis of a therapeutic monoclonal antibody, trastuzumab, using a BIOshell™ A400 Protein C4 column.
Correlation of FcR Affinity Chromatography with Glycan Pattern and ADCC Activity of a Therapeutic Antibody
Fast assessment of antibody-dependent cell-mediated cytotoxicity (ADCC) and glycoform pattern of a therapeutic antibody rituximab by FcγRIIIa affinity chromatography.
A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MS
The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.
Robust, load independent viral clearance in monoclonal antibody purification with Natrix® Q chromatography membrane
Membrane adsorbers are a cost-effective, robust, single-use solution for monoclonal antibody polishing
O Linked Glycans
O-Linked glycans are usually attached to the peptide chain through serine or threonine residues. O-Linked glycosylation is a true post-translational event and does not require a consensus sequence. The most common type of O-linked glycans contain an initial GalNAc residue
Absolute Quantification of Serum Proteome
Absolute Quantification (Protein-AQUA™™) is a targeted quantitative proteomics technique that exhibits robust efficacy and is being increasingly utilized for a wide variety of quantitative proteomics studies.
Enzymes for Antibody Characterization
Antibodies now make up the fastest growing category of therapeutic drugs
Quantification of Host Cell Proteins
Residual presence of host cell proteins (HCPs) in recombinant therapeutic products has considerable clinical safety risks associated with a potential immunological response in patients.
Optimising the LC-MS Analysis of Biomolecules
We present an article concerning optimising the LC-MS analysis of biomolecules.
Middle-Up Analysis of Antibodies by Reversed-Phase Chromatography
Comparative analysis of different columns in resolving medium-sized fragments of monoclonal antibodies, after digestion using dithiothreitol (DTT) or IdeS (a protease), by Reversed-Phase Chromatography.
Improving the Chromatographic Separation of DMB-Labeled Sialic Acids for the Comparison of Biosimilars to Reference Materials
A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.
Three Methods for Critical Quality Attribute Determination of Monoclonal Antibodies
Step-by-step workflows for the intact mass analysis, peptide mapping, and N-glycan analysis of the monoclonal antibody― adalimumab, for an accurate characterization of the critical quality attributes (CQAs) to ensure drug safety and efficacy. Read more.
Analysis of Oligonucleotides by SEC-MALS
Separation and identification of oligonucleotides differing by one base in length using Size Exclusion Chromatography combined with multi-angle light scattering (SEC-MALS) detector.