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Phosphorylation Analysis Tools
Phosphorylation is an important covalent post-translational modification (PTM) in cell signalling pathways. Protein phosphorylation is the reversible addition of a phosphate group to a protein or small molecule catalysed by protein kinases. Approximately one third of the 30,000 proteins encoded
Post Translational Modification
Post-translational modifications such as glycosylation, phosphorylation, and sulfation, to name a few, serve many functions. As a result, the analysis of proteins and their post-translational modifications is particularly important for the study of diseases where multiple genes are known to
Designing Peptides
When selecting peptides for custom synthesis, several important factors should be considered during the design process. These considerations include sequence length, solubility and sequence stability.
Glycoprotein Deglycosylation
Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.
Strategies for Deglycosylating N-Linked Glycans
Explore various strategies for deglycosylating N-linked glycans involving PNGase F, PNGase A (Glycopeptidase A), and even native and sequential deglycosylation with endoglycosidases like Endoglycosidase H, Endoglycosidase F, and exoglycosidases.
N-Linked Glycans Overview
N-linked glycosylation, modification, and degradation
Peptidoglycan Structure, Biosynthesis and Function
The basic structure of peptidoglycan (PGN) contains a carbohydrate backbone of alternating units of N-acetylglucosamine (GlcNAc) and Nacetylmuramic acid, with the N-acetylmuramic acid residues cross-linked to peptides.
EX-CELL® Glycosylation Adjust (GAL+)
Our protein quality supplement, EX-CELL® Glycosylation Adjust (Gal+), provides customers with a novel chemically defined product which targets glycosylation attributes.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification
Atto Dyes and Tracy Dyes for Fluorescent Protein Labeling
We offer protein labeling kits based on two types of fluorescent dyes, the Atto dyes and the Tracy dyes. Both series of kits provide an easy and reliable way to fluorescently label purified proteins, enzymes, and antibodies.
DMABA Derivatization: Aminophospholipid Detection Multiplexing
In this video Dr. Robert Murphy, professor at University of Colorado - Denver, discusses DMABA Derivatization: Aminophospholipid Detection Multiplexing.
Protein Prenyltransferases
We offer many products related to protein prenyltransferases for your research needs
Cleavage and Purification of GST-Tagged Protein Bound to GSTrap
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Glycan Labeling
Structural modifications of proteins are essential to living cells. When aberrantly regulated they are often the basis of disease. Glycans are responsible for much of the structural variation in biologic systems, and their representation on cell surfaces is commonly called
ChemMatrix® Resin for solid phase peptide synthesis
ChemMatrix® is a proprietary, 100% PEG (polyethylene glycol) based resin from PCAS BioMatrix.
Cell and Organelle Labeling with Fluorescent Antibodies
The Human Protein Atlas Program has carefully selected three different human cell lines, A-431 epidermoid carcinoma, U-251 MG glioblastoma and U-205 osteosarcoma, for organelle mapping of the proteome. As Prestige Antibodies are studied by immunofluorescence (IF) staining, three well-characterized organelle
Solid Phase Synthesis
Peptides are manufactured using solid phase FMOC or BOC chemistry methodologies on a PEG-Polystyrene support resin. Upon synthesis completion, side chain protecting groups are removed and the peptides are simultaneously cleaved from the resin. The cleaved and deprotected
Methods and Matrices for Mass Spectrometry of Glycans
Glycosylation is known to have profound influence on various physiochemical, cellular and biological functions of proteins. Alterations in this modification are known to affect the immune system and have been associated with various pathological states such as cancer, rheumatoid arthritis
Azide Building Blocks for Chemical Ligation
Chemoselective ligation strategies are a key success factor for chemical biology research. Ligation techniques open pathways to fully synthetic large peptides and even proteins.
ChiPros Chiral Amines
Chiral amines play an important role in stereoselective organic synthesis. They are used directly as resolving agents, building blocks, or chiral auxiliaries.
Mass Spectrometry
What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.
Fluorescent Labeling of Peptides
Fluorescent Labeling of Peptides
Unnatural Amino Acids
Unnatural amino acids, the non-proteinogenic amino acids that either occur naturally or are chemically synthesized, are becoming more and more important as tools for modern drug discovery research.
Proline Derivatives and Analogs
Proline analogues are promising candidates for tuning the biological, pharmaceutical, or physicochemical properties of naturally occuring, as well as de novo designed, linear, and, cyclic peptides.
Gold Nanoparticles: Properties and Applications
Gold (Au) nanoparticles have tunable optical and electronic properties and are used in a number of applications including photovoltaics, sensors, drug delivery & catalysis.
Troubleshooting Strategies in GST-tagged Protein Purification
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.
Histone Modification and Chromatin Remodeling | Epigenetics
Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.
Biotinylated Peptides
Our Streptavidin HC (High-Capacity) multiwell plates are prepared with a highly-porous, high-density streptavidin coating. Streptavidin has similar biotin-binding characteristics as avidin. Streptavidin can bind 4 moles of biotin per mole of protein with high selectivity and affinity (Kd~10-15). Unlike avidin
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