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HPLC Analysis of Adenosine Monophosphate (AMP) and Adenosine Triphosphate (ATP) on SeQuant ZIC-HILIC
Separation of AMP (adenosine monophosphate); ATP (adenosine triphosphate)
Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC
Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.
Famciclovir Tablets USP Monograph Method Using a Purospher™ STAR RP-8 endcapped HPLC Column and UV Detection
Following the new USP monograph for Famciclovir Tablets, Purospher™ STAR RP-8 endcapped HPLC columns can be used to monitor organic impurities in Famciclovir tablet formulations. Famciclovir is an antiviral drug indicated for the treatment of herpes zoster, herpes simplex virus
Assay and Organic Impurity Profiling of Apixaban Using an Ascentis® Express C18 Column and UV Detection
Apixaban is sold under the tradename Eliquis and at present no pharmacopeial monographs are available for Axpixaban using either an assay and/or organic impurity profiling method. A newly developed and validated HPLC-UV method using a column with C18 solid core
Universal Hydrophilic Syringe Filters: Millex® Hydrophilic PTFE
Millex® hydrophilic PTFE syringe filters boast broad chemical compatibility and are HPLC-certified, guaranteeing low extractables. With their robust performance, these filters can be used for a variety of samples without the risk of membrane failure or sample contamination.
High-Resolution U/HPLC Separation of Monoclonal Antibodies with the BIOshell™ IgG 1000 Å Diphenyl
BIOshell™ IgG 1000 Å Diphenyl columns are perfectly suitable for the separation of monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights ≤ 500 kDa with alternative selectivity to C4 and C18 column chemistries to ensure maximum
Dyestuff Analyses on Historic Objects by LC-ESI-MS/MS Using a Superspher RP-18 HPLC Column
In this study, LC-ESI-MS/MS under reversed phase conditions using a Superspher RP-18 column is applied to analyze dyestuffs of a reddish historic textile from South America.
UHPLC Analysis of Nucleosides on Supel™ Carbon LC Column: Performance Comparisons with Competitor
Ultra-high performance liquid chromatographic (UHPLC) separation of 12 nucleosides using Supel™ Carbon LC column in 15 minutes for use in identification and quantitation of nucleoside biomarkers.
Determination of Tramadol in Urine using LC-MS/MS
This article presents an LC-MS/MS method for the determination of tramadol in urine samples using reversed-phase LC-MS/MS aided by isotope internal standards.
UHPLC Analysis of Paraquat and Diquat on Supel™ Carbon LC
Ultra-high performance liquid chromatographic (UHPLC) separation of polar herbicides, paraquat and diquat using SupelTM Carbon LC column with baseline resolution, excellent peak shape, and good lot-to-lot reproducibility.
UHPLC Analysis of Conjugated Estrogens on Ascentis ® Express C18
Separation of Estriol 3-(β-D-glucuronide) sodium salt; β-Estradiol 3-(β-D-glucuronide) 17-sulfate dipotassium salt; Estriol 3-sulfate sodium salt; β-Estradiol 3,17-disulfate dipotassium salt, ≥95%; β-Estradiol 17-(β-D-glucuronide) sodium salt; β-Estradiol 3-(β-D-glucuronide) sodium salt; Estrone 3-(β-D-glucuronide) sodium salt; β-Estradiol 3-sulfate sodium salt, ≥93%; Estriol, ≥97%; Estrone
Determination of Ephedrine HCl and Pseudoephedrine HCl in Xiao’er Kechuanling Oral Solution using a Discovery® HS C18 Column
Developing an HPLC method for quantifying ephedrine HCl and pseudoephedrine HCl in traditional Chinese medicine (TCM) with a Discovery® C18 column to report: chromatographic data, linearity, specificity and repeatability, LOD and LOQ.
Determination of Chlorogenic acid and Baicalin in compounded Lonucerae Japonicae Flos with Purospher® STAR RP-18e
Extraction and analysis of chlorogenic acid and baicalin active ingredients in traditional Chinese medicine (TCM) according to Chinese pharmacopeia monograph by HPLC with Purospher® RP-18e column
HPLC Analysis of Sugars by HILIC Chromatography using Ascentis® Express OH-5 Column with ELSD Detection
Separation of Dextrose, Pharmaceutical Secondary Standard; Certified Reference Material; Sucrose; Maltitol, Pharmaceutical Secondary Standard; Certified Reference Material
Application Note – Peroxidase in Vegetables
Application Note: Peroxidase in vegetables – Visual determination after conversion of a specific substrate.
HPLC Analysis of Proteins on BIOshell™ A400 Protein C4, Rapid Separation at Elevated Temperature
For use as a marker in SDS-PAGE; lactate dehydrogenase; contaminant; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
UHPLC Analysis of Ephedrine and Related Compounds on Supel™ Carbon LC Column
Ultra-high performance liquid chromatographic (UHPLC) separation of ephedrine and its three structural analogs based on polar retention effect on graphite (PREG) mechanism using Supel™ Carbon LC column.
HPLC Analysis of Sugars on Ascentis® Si
Separation of Sucrose; Maltose; Maltotriose
Applications of Newly Developed 2.7 μm Porous Graphitic Carbon Particles in U/HPLC
Polar graphitic carbon (2.7 μm) based Ultra/High-performance liquid chromatographic separation of four highly polar pesticides, two polar herbicides―paraquat & diquat, and Vitamin D and its metabolites.
HPLC Tips & Tricks: Increase Your HPLC/UHPLC Method Sensitivity
Tips & tricks to improve your chromatographic method sensitivity by reducing baseline noise and/or making suggested improvements to increase signal intensity.
HPLC Analysis of Pain Relievers on Ascentis® Express C18
Separation of Acetaminophen, meets USP testing specifications, 98.0-102.0%, powder; Caffeine, meets USP testing specifications, anhydrous; Benzoic acid, meets USP testing specifications; Aspirin, meets USP testing specifications
HPLC Analysis of Proteins on TSKgel® BioAssist S
Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis;
HPLC analysis of Letrozole and its impurities by following the USP Guidelines Using a Purospher® STAR RP18e Column and UV Detection
An application note concerning the HPLC analysis of letrozole and its impurities by following the USP43-NF38 Guidelines using a Purospher® STAR RP18e Column.
Determination of Buprenorphine and Norbuprenorphine in Urine Using LC-MS/MS
Determination of Buprenorphine and Norbuprenorphine in Urine Using LC-MS/MS
HPLC Tips & Tricks: Mobile Phase Preparation Part 2 - Buffers
Learn the tips and tricks of selecting and preparing a suitable mobile phase buffer for your HPLC separations and method developments for samples that contain ionizable compounds.
Determination Paeonol Morroniside Loganin Liuwei Dihuang Oral Solution
Method development for the extraction and analysis of paeonol, morroniside, loganin active ingredients in traditional Chinese medicine (TCM) according to Chinese pharmacopeia monograph using HPLC-UV
LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions)
The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity
Ascentis® Express C8 U/HPLC Columns
When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.
LC/MS Analysis of Nicotine and Metabolites on Kromasil® Eternity™ C18 (Analyte Set 1)
Separation of (+)-Anabasine hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material; S(−)-Nicotine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material; (S)-N-Nitrosoanabasine (NAB) solution, 1.0 mg/mL in methanol, ampule
Ascentis® Express RP-Amide HPLC Columns
Sigma-Aldrich.com presents an article about innovative particle technology and RP-Amide bonding chemistry provide the benefits of speed, resolution, selectivity, aqueous stability, and improved peak shape for bases.
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