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Transfection and gene editing

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Calcium Phosphate Transfection Kit Protocol
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
Yeast Transformation Kit
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)
Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents
Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents
X-tremeGENE™ 9 DNA Transfection Reagent Protocol
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Lentiviral Production Using X-tremeGENE HP Transfection Reagent
Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.
Genome Editing in Plants with CRISPR/Cas9
ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.
Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®
This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.
Preparation of Competent Cells and Transformation with pGEX DNA
This page describes preparation of competent cells and transformation with pGEX DNA.
Escort™ III Transfection Reagent Protocol
The product bulletin providin detailed use protocol for easy DNA transfection.
Escort™ IV Transfection Reagent Protocol
Product manual provides detailed protocol for easy DNA transfection.
Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids
This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells
Procedure for Preparation of Cationic Lipid/Oligomer Complex - Avanti® Polar Lipids
This procedure describes the preparation of Cationic Lipid/Oligomer Complex.
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
Lentiviral Transduction Protocol
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
X-tremeGENE™ HP DNA Transfection Reagent Protocol
Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
CRISPR Lentiviral Screening: Antibiotic Selection
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Co-transfection of Plasmid DNA
Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.
Liposome Preparation - Avanti® Polar Lipids
Lipid vesicle preparation involves hydration and sizing steps, applicable regardless of lipid composition.
FACS Titration of Lentivirus Protocol
FACS sorts cells based on light scattering and fluorescence for objective cell analysis.
Universal Transfection Reagent Protocol
Universal Transfection Reagent enables efficient nucleic acid delivery into various cells, compatible with different cell culture conditions.