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Amicon® Stirred Cells
User guide for Amicon® Stirred cells including features, operation, specifications, and troubleshooting.
Rat Dermal Fibroblasts (RDF) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Enzymatic Assay of Phytase (EC 3.1.3.8)
Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure.
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
Enzymatic Assay of Urease from Jack Beans
To standardize a procedure for the enzymatic assay of Urease, from Jack Beans
Enzymatic Assay of Cholesterol Oxidase
This procedure applies to products that have a specification for the enzymatic activity of cholesterol oxidase. This assay is NOT to be used to assay Cholesterol Oxidase from Schizophyllum commune (Discontinued Product Number C7274) and from Brevibacterium sp. (Discontinued Product
CRISPR Cas 9 Nuclease RNA-guided Genome Editing
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
Chromatin Isolation by RNA Purification (ChIRP) Protocol
Protocol for Chromatin Isolation by RNA Purification (ChIRP) Protocol
Handling and Storage Protocol for Synthetic Peptides
One of the most challenging aspects of working with synthetic peptides is determining the best solvent in which to dissolve the peptide. General guidelines for proper storage, handling and dissolving your synthetic peptide are provided.
Neonicotinoids
Learn more about Neonicotinoids - active substances used in plant protection products to control harmful insects.
Rapid DNA Ligation Kit Protocol
Rapid DNA Ligation Kit Protocol
GC Analysis of Oxygen, Nitrogen, and Carbon Dioxide on Carboxen®-1010 PLOT
GC Analysis of Oxygen, Nitrogen, and Carbon Dioxide on Carboxen®-1010 PLOT
Enzymatic Assay of β-AMYLASE (EC 3.2.1.2)
Enzymatic Assay of β-AMYLASE (EC 3.2.1.2)
Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
SnAP Reagents
Saturated N-heterocyclic building blocks or SnAP Reagents are of growing importance for the convenient synthesis of medium-ring saturated N-heterocycles, including bicyclic and spirocyclic structures. SnAP reagents are stable and readily available and can be coupled with widely available aromatic, heteroaromatic
Enzymatic Assay of Esterase
Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
MSTFA/MSTFA-d9 Derivatization of Amphetamine for GC/MS Detection and Identification
Example displays how MSTFA/MSTFA-d9 derivatization of amphetamine provides valuable information in mass spectra and chromatograms using SLB-5ms GC column.
Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
Enzymatic Assay of Proteinase K (EC 3.4.21.64)
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol
GC Analysis of Total Petroleum Hydrocarbons (TPH) on SLB®-5ms
Separation of Hexane; Heptane; Octane; Nonane; Decane; Undecane; Dodecane; Tetradecane; Hexadecane; Octadecane; Eicosane; Tetracosane; Octacosane; Dotriacontane; Hexatriacontane; Tetracontane; Tetratetracontane
Saliva DNA Extraction & WGA Amplification Protocol
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
Enzymatic Method for Determining Galactose (Galactose Assay)
This assay protocol is suitable for the colorimetric/fluorometric detection of Galactose and Lactose in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Galactose Assay Kit.
Performing a Purification of Antibody Fragments with Capto™ L Products
This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.
radiello® Diffusive Air Sampling Application - Volatile Organic Compounds (VOCs) Thermally Desorbed
Volatile organic compounds are trapped by adsorption and recovered by thermal desorption, analysis is performed by capillary gas chromatography and FID or MS detection.
GenElute™ RNA/DNA/Protein Plus Purification Kit
GenElute RNA/DNA/Protein Plus Purification Kit provides a rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
Liberase™ TL Research Grade Protocol & Troubleshooting
Liberase™ TL Research Grade Protocol & Troubleshooting
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Genomic DNA Purification from Sample Applied to FTA Cards
This page shows genomic DNA purification from sample applied to FTA cards from Cytiva.
Genomic DNA Purification using illustra™ GenomicPrep Mini Spin Kits
This page shows Genomic DNA purification using illustra™ genomicPrep Mini Spin Kits from Cytiva.
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