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Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns

His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superﬂow medium. Each column provides simple manual puriﬁcation of up to 15 mg of histidine-tagged proteins.

HPLC Analysis of Adenosine Monophosphate (AMP) and Adenosine Triphosphate (ATP) on SeQuant ZIC-HILIC

Separation of AMP (adenosine monophosphate); ATP (adenosine triphosphate)

Iscove's Modified Dulbecco's Media (IMDM) Formulation

Lipoic (Thioctic) Acid, in Cell Culture

How lipoic acid (thioctic acid) and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

L-15 Media Formulation

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages.

F-12 Coon's Modification Formulation

C-O Cross-Coupling of Activated Aryl and Heteroaryl Halides with Aliphatic Alcohols

JosiPhos CyPF-tBu and palladium give catalyst for alkoxylation of activated heteroaryl halides with primary, secondary, and tertiary alcohols

Knoevenagel Condensation Reaction

Knoevenagel Condensation is an organic reaction named after Emil Knoevenagel. It is a classic C-C bond formation reaction and a modification of the Aldol Condensation.

1-hydrosilatrane

As a versatile hydride reagent, 1-hydrosilatrane can be used in reduction reactions to synthesize alcohols, amines, their chiral counterparts, and esters from aldehydes/ketones.

Biginelli Reaction

The Biginelli Reaction is an acid-catalyzed, three-component reaction between an aldehyde, b-ketoester, and urea that produces tetrahydropyrimidones, which have potential pharmaceutical applications.

ADCC Assay Using BioIVT Cryopreserved NK Cells

ADCC assay performance and reproducibility using ready-to-use, cryopreserved NK effector cells, A549 target cells expressing a luciferase reporter gene, and anti-EGFR antibody.

Peptide Labeling

Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.

Fibroblast Growth Factor Family (FGF)

Fibroblast growth factors in cell culture and various growth factors for your research

Ellman's Sulfinamides

Ellman's sulfinamide is available in both enantiomeric and racemic forms for your research. This versatile and useful auxiliary has found extensive use both in academics and industry.

Heterocycle and Cyclic Peptide Formation with N-(isocyamino)triphenylphosphorane (Pinc)

Isocyanides are widely used reagents in organic synthesis, with applications ranging from materials science to drug discovery.

Oleic Acid in Cell Culture

How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Hematopoietic Cytokines

Read article on hematopoietic cytokines and hematopoiesis

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

Copper in Cell Culture

Importance and uses of copper in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO), cell cultures

Mammary Epithelial Cell Growth Medium

Serum-free Mammary Epithelial Cell Growth Media optimized for the in vitro cultivation of epithelial cells from the mammary gland. Protocols for handling, cell passaging, media, and product use.

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

Linolenic Acid in Cell Culture

Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Cell Death Troubleshooting in Cell Culture

Here we discuss causes of cell death, and how to assess and prevent these causes.

Citrate Serum-Free Medium Supplement

Citrate is one of many cell culture components that affects the performance of serum-free, protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins such as monoclonal antibodies.

Cell Analysis Best Practices

How do you assess the state of your cell culture? We’ve got some best practices to share.

Identification of Cell Culture Media with Raman Spectroscopy

Identification of cell culture media samples by traditional LC methods, such as amino acid or vitamin analysis, has high costs, and requires significant analytical expertise and laboratory space.

Common Cell Culture Problems: Precipitates

Common Cell Culture Problems: Precipitates -- When contamination is ruled out, turbidity in cell culture media is often explained by the precipitation of metals, proteins, and other media components. Precipitates can be harmful to cell health, as they may alter

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

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