Merck
  • High-affinity mutant Interleukin-13 targeted CAR T cells enhance delivery of clickable biodegradable fluorescent nanoparticles to glioblastoma.

High-affinity mutant Interleukin-13 targeted CAR T cells enhance delivery of clickable biodegradable fluorescent nanoparticles to glioblastoma.

Bioactive materials (2020-05-15)
Gloria B Kim, Virginia Aragon-Sanabria, Lauren Randolph, Hali Jiang, Joshua A Reynolds, Becky S Webb, Achuthamangalam Madhankumar, Xiaojun Lian, James R Connor, Jian Yang, Cheng Dong
ABSTRACT

Glioblastoma (GBM), the deadliest form of brain cancer, presents long-standing problems due to its localization. Chimeric antigen receptor (CAR) T cell immunotherapy has emerged as a powerful strategy to treat cancer. IL-13-receptor-α2 (IL13Rα2), present in over 75% of GBMs, has been recognized as an attractive candidate for anti-glioblastoma therapy. Here, we propose a novel multidisciplinary approach to target brain tumors using a combination of fluorescent, therapeutic nanoparticles and CAR T cells modified with a targeted-quadruple-mutant of IL13 (TQM-13) shown to have high binding affinity to IL13Rα2-expressing glioblastoma cells with low off-target toxicity. Azide-alkyne cycloaddition conjugation of nanoparticles to the surface of T cells allowed a facile, selective, and high-yielding clicking of the nanoparticles. Nanoparticles clicked onto T cells were retained for at least 8 days showing that the linkage is stable and promising a suitable time window for in vivo delivery. T cells clicked with doxorubicin-loaded nanoparticles showed a higher cytotoxic effect in vitro compared to bare T cells. In vitro and in vivo T cells expressing TQM-13 served as delivery shuttles for nanoparticles and significantly increased the number of nanoparticles reaching brain tumors compared to nanoparticles alone. This work represents a new platform to allow the delivery of therapeutic nanoparticles and T cells to solid tumors.

MATERIALS
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Brand
Product Description

Corning® Transwell® polycarbonate membrane cell culture inserts, 6.5 mm Transwell with 5.0 μm pore polycarbonate membrane insert, TC-treated, w/lid, sterile, 48/cs
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Histopaque®-1077, sterile-filtered, density: 1.077 g/mL
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Hydrocortisone solution, 50 μM, sterile-filtered, BioXtra, suitable for cell culture
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Fluoroshield with DAPI, histology mounting medium
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Triton X-100, laboratory grade
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Goat Serum Donor Herd, USA origin, sterile-filtered, suitable for cell culture