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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Supelco
Diethylene glycol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
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Acetone, ≥99.5%, ACS reagent
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Sodium chloride solution, 0.85%
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Methanol, suitable for NMR (reference standard)
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Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
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Sodium chloride, random crystals, 99.9% trace metals basis
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Piperidine, ≥99.5%, purified by redistillation
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Acetone, natural, ≥97%
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Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
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Acetone, ≥99%, FCC, FG
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Methanol, low water for titration
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Tetraethyl orthosilicate, ≥99.0% (GC)
Supelco
(±)-Epichlorohydrin, analytical standard
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Diethylene glycol, BioUltra, ≥99.0% (GC)
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Diethylene glycol, analytical standard
Sigma-Aldrich
Formaldehyde solution, meets analytical specification of USP, ≥34.5 wt. %
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Chloroform, puriss. p.a., ACS reagent, ≥99.8% (chloroform + ethanol, GC)
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Chloroform, analytical standard
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Sodium chloride, reference material for titrimetry, certified by BAM, >99.5%
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2-Mercaptoethanol, BioUltra, Molecular Biology, ≥99.0% (GC)
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Diethylene glycol, puriss. p.a., ≥99.0% (GC), colorless
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Formaldehyde solution, Molecular Biology, BioReagent, ≥36.0% in H2O (T)
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N-Ethyldiisopropylamine, ≥98.0%
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Sodium chloride, BioUltra, Molecular Biology, ≥99.5% (AT)
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Sodium chloride, tested according to Ph. Eur.
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1-Octanol, analytical standard
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Sodium chloride solution, BioUltra, Molecular Biology, ~5 M in H2O
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Methanol, analytical standard
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Tetrahydrofuran, analytical standard