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Supelco Preparative HPLC products for Pharmaceutical Development and Production

Untargeted profiling of Glycyrrhiza glabra extract with 2D LC MS

An RP-LC×RP-LC-PDA-MS method for the metabolic profiling of G. glabra extract using the MSG approach for enhanced metabolic coverage.

Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum

Novel sample prep technique coupled with the unique selectivity of the Ascentis Express F5 column enables a fast and simplified bioanalytical method for associated vitamin D metabolites.

Mixed-Mode SPE Improves Extraction of Pharmaceutical Compounds from Biological Fluids

Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

Solid Phase Extraction (SPE) Overview

Solid phase extraction method extracts, partitions, and adsorbs components from liquid samples onto stationary phase resin.

Solid Phase Extraction for Aflatoxin Analysis

Supel Tox AflaZea SPE improves analyte recovery with less sample volume, while HPLC confirms effective elimination of interferences.

Supel™-Select Polymeric SPE

Supel-Select HLB SPE - a hydrophilic modified styrene based polymer developed for the solid phase extraction of a broad range of compounds from aqueous samples.

Resolves Difficult Sample Matrix Problems

In the flavor and fragrance industry, analytical chemists are often required to isolate key ingredients, off-flavor components, or trace organic compounds from their sample matrices without altering their structure or composition.

Karl Fischer Titration Tips: Water Content Measurement

Methods including internal extraction, external extraction, and Karl Fischer oven ensure accurate water content determination by titration.

Coulometric Titration - Faster and More Efficient New Aquastar^® Coulometric Reagent for Karl Fischer Water Determination

New Aquastar® Karl Fischer Anolyte for precise and reproducible determination of low water content in the range of 10 ppm to 10000 ppm for cells without a diaphragm.

Advanced Cell Culture Technology for Generation of In Vivo-like Tissue Models

Sophisticated models are developing as 3D cell culture technology becomes established and accepted as a means of creating more physiologically relevant cell-based assays.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

5 Reasons Cancer Researchers Adopt 3D Cell Culture: A Review of Recent Literature

Numerous cancer model systems are available to investigate disease mechanisms and to screen therapies. While all of the models have contributed critical information about cancer biology.

High-Throughput Drug Screening using TrueGel3D® HTS Hydrogel Plates

Compound cytotoxicity screening assays with 3D cell cultures using TrueGel3D® HTS Hydrogel Plates including dose response curves, IC50 determination, and spheroid killing assay data.

3D Pluripotent Stem Cells: Culture & Differentiation

3D cell culture enhances pluripotent stem cell models of embryo development, providing insights into differentiation.

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Utilization of TrueGel3D™ Hydrogels to Study Fibroblast Spreading

Fibroblasts that are present in the stroma secrete glycoproteins and polysaccharides to form extracellular matrix. They maintain the structural integrity within the connective tissue and play a key role in cancer, chronic obstructive pulmonary diseases, idiopathic pulmonary fibrosis, scleroderma and

Autophagy Assays

Cell based autophagy assays including live cell LC3 GFP/RFP lentiviral biosensors, kits for autophagy detection and autophagy activators and inhibitors.

Caco-2 Intestinal Drug Transporter Models for More Predictive Drug Absorption

Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.

Hepatotoxicity/Cytotoxicity Assays

Hepatotoxicity/cytotoxicity assays measure cell death due to test compounds in various formulations.

Size-based Immunomonitoring using the Scepter™ 3.0 Handheld Cell Counter

The Scepter™ 3.0 Cell Counter, when used in conjunction with a 40 μm aperture sensor, can accurately count and precisely size a wide range of cell types and particles. This application note demonstrates two examples of experiments using the instrument’s

Validated Cell Types for Millicell^® DCI Digital Cell Imager

Cell validation data using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.

Validated Human Cell Types for Millicell^® DCI Digital Cell Imager

Data for validated human cell lines using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.

EX-CELL^® Advanced™ CHO Fed-Batch System – Bioprocessing Technology Institute, Singapore Case Study

In this study, nine commercially available, chemically defined media and feed combinations from different vendors were assessed for producing monoclonal antibody in Chinese hamster ovary (CHO) cells.

Impact of DMSO And Freezing Technique in Developing a High Cell Density Cryopreservation Process for Upstream Bioprocessing

Understand how considerations such as DMSO concentrations and freezing techniques are key in the successful implementation of high cell density cryopreservation (HCDC).

Modified Amino Acids

Modified amino acids are inhouse manufactured amino acid derivatives with specific properties enabling the intensification of cell culture processes.

Frequently Asked Questions About Gamma-Irradiated FBS (Serum) and Other Products for Cell Culture

Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.

Human Serum Albumin (HSA)

Learn more about the cell culture supplements human serum albumin (HSA) and recombinant human serum albumin (rHSA). Explore their applications in cell culture and beyond.

Mycoplasma Clearance Performance of Stericup^® and Steritop^® Filters

Evaluation of mycoplasma removal by sterilizing filtration using 0.1 µm PES and PVDF filters for cell culture media and buffer preparation.

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