HomePolymerase Chain Reaction ApplicationsOligoEvaluator™ for Tm Calculation & Primer Analysis

OligoEvaluator™ for Tm Calculation & Primer Analysis

OligoEvaluator™ for DNA or RNA Primers

We are pleased to offer OligoEvaluator™, our online oligonucleotide sequence calculator that provides primer analysis values for PCR:

  • Base count
  • Molecular weight
  • Extinction coefficient
  • Oligo type
  • µg/OD at 260 nm
  • Length (base pairs)
  • Primer melting temperature Tm (°C)
  • GC content %
  • GC clamp
  • Run length (bp)
  • Secondary structure
  • Primer dimer check
  • BLAST sequence link

OligoEvaluator™ is easy to use: select DNA or RNA, paste your sequence, and click calculate for the OligoEvaluator™ tool to return values. All reported properties are available for export to a convenient Excel template.

Compute Oligo Properties, Resuspension & Dilution

The OligoEvaluator™ consists of three modules. Choose analysis to look at the complete properties of up to 10 primers at a time. Resuspension and Dilution are separate calculator modules within OligoEvaluator™.

Calculator Advanced Features

Reaction Conditions

Definitions and values used in the OligoEvaluator™ calculator

How do you calculate the annealing temperature of a primer?

The OligoEvaluator™ provides melting temperature for oligos. Just enter you sequence in the calculator Analysis module, and you'll find the Tm reported in the seventh column. Annealing temperature should usually be several degrees below the Tm value. You can find more detailed information about melting temperature in our article about Oligonucleotide Melting Temperature.

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