OligoEvaluator™ for T_m Calculation & Primer Analysis

OligoEvaluator™ for DNA or RNA Primers

We are pleased to offer OligoEvaluator™, our online oligonucleotide sequence calculator that provides primer analysis values for PCR:

• Base count
• Molecular weight
• Extinction coefficient
• Oligo type
• µg/OD at 260 nm
• Length (base pairs)
• Primer melting temperature T_m (°C)
• GC content %
• GC clamp
• Run length (bp)
• Secondary structure
• Primer dimer check
  
• BLAST sequence link

OligoEvaluator™ is easy to use: select DNA or RNA, paste your sequence, and click calculate for the OligoEvaluator™ tool to return values. All reported properties are available for export to a convenient Excel template.

Compute Oligo Properties, Resuspension & Dilution

The OligoEvaluator™ consists of three modules. Choose analysis to look at the complete properties of up to 10 primers at a time. Resuspension and Dilution are separate calculator modules within OligoEvaluator™.

Reaction Conditions

Definitions and values used in the OligoEvaluator™ calculator

How do you calculate the annealing temperature of a primer?

The OligoEvaluator™ provides melting temperature for oligos. Just enter you sequence in the calculator Analysis module, and you'll find the T_m reported in the seventh column. Annealing temperature should usually be several degrees below the T_m value. You can find more detailed information about melting temperature in our article about Oligonucleotide Melting Temperature.