ECACC Laboratory Handbook 4th Edition
Figure 1.Testing Cells for Mycoplasma Contamination
Detection of mycoplasma by culture isolation methods is the reference method of detection and has a theoretical level of detection of 1 colony-forming unit (cfu). However, there are some strains of mycoplasma that are non-cultivable (certain strains of Mycoplasma hyorhinis). The method is suitable for the detection of mycoplasma in both cell cultures and cell culture reagents and results are obtained within 4 weeks. Mycoplasma colonies observed on agar plates have a ‘fried egg’ appearance.
* Available from the National Collection of Type Cultures (NCTC)
All positive control agar plates and broths show evidence of mycoplasma by typical colony formation on agar plates and usually a color change in broths. All negative control agar plates and broths show no evidence of mycoplasma.
Test agar plates infected with mycoplasma show typical colony formation.
The test agar plates show no evidence of mycoplasma.
Figure 2.Detection of mycoplasma
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