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861065

Sigma-Aldrich

Azure II

powder

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Synonym(s):
3-Chloro-6-hydroxy-2-Pyridinecarboxylic acid, Azur II
Linear Formula:
C16H18N3S · C15H16N3S ·2 Cl
CAS Number:
Molecular Weight:
625.68
Colour Index Number:
52010/52015
PubChem Substance ID:
NACRES:
NA.47

description

mixture of Azure B and Methylene Blue in equal amounts.

Quality Level

form

powder

solubility

water: soluble

λmax

657 nm

ε (extinction coefficient)

≥1500 at 652-658 nm in water at 0.003 g/L
≥400 at 243-249 nm in water at 0.003 g/L
≥800 at 288-294 nm in water at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].CNc1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](\C)C.CN(C)c4ccc5N=C6C=C\C(C=C6Sc5c4)=[N+](\C)C

InChI

1S/C16H18N3S.C15H15N3S.2ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;;/h5-10H,1-4H3;4-9H,1-3H3;2*1H/q+1;;;/p-1

InChI key

YOWZJZJLXUQHGF-UHFFFAOYSA-M

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1 of 4

This Item
A2918861049227935
Azure II powder

861065

Azure II

Azure A chloride certified by the Biological Stain Commission

A2918

Azure A chloride

Azure A chloride certified by the Biological Stain Commission, Dye content 70 %

861049

Azure A chloride

Azure B prepared by direct synthesis

227935

Azure B

form

powder

form

powder

form

powder

form

powder

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

λmax

657 nm

λmax

-

λmax

633 nm

λmax

648 nm

ε (extinction coefficient)

≥1500 at 652-658 nm in water at 0.003 g/L, ≥800 at 288-294 nm in water at 0.003 g/L, ≥400 at 243-249 nm in water at 0.003 g/L

ε (extinction coefficient)

-

ε (extinction coefficient)

≥11000 at 242-246 nm in H2O at -1, ≥26000 at 287-291 nm in H2O, ≥34000 at 620-634 μm in H2O

ε (extinction coefficient)

≥13000 at 241-247 nm, ≥36000 at 287-293 nm, ≥67000 at 638-644 nm

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

Application

Azure II has been used in the Richardson′s stock solution for the staining of lysosomal glycogen. It has also been used in the periodic acid schiff staining procedure for the staining of glycogen, nuclei and cytoplasm.

Biochem/physiol Actions

Azure II complex is used for the staining of nuclei, goblet cells, striated muscle, endothelial cells, smooth muscle, cytoplasm, lipid droplets, red blood cells, glycogen, collagen, cartilage, mucus granules, elastin and leukocytes.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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