1.00327

Hydrogen chloride solution

in ethanol, for microscopy

• Synonym(s): Hydrogen chloride – ethanol solution, Hydrogen chloride solution, Hydrochloric acid in ethanol
• Linear Formula: HCl · CH₃CH₂OH
• CAS Number: 7647-01-0
• Molecular Weight: 36.46
• NACRES: NA.21
• UNSPSC Code: 41116130
• MDL number: MFCD00011324
• Beilstein/REAXYS Number: 1098214

Properties

• Product Name: Hydrogen chloride solution, in ethanol, for microscopy
• InChI key: VEXZGXHMUGYJMC-UHFFFAOYSA-N
• InChI: 1S/ClH/h1H
• SMILES string: Cl
• form: liquid
• IVD: for in vitro diagnostic use
• technique(s): microbe id | staining: suitable
• pH: 1.0 (20 °C in H₂O)
• transition temp: flash point 21 °C
• density: 0.90 g/cm³ at 20 °C
• application(s): clinical testing; diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• Quality Level: 400

Description

Application

For microscopy. Ziehl-Neelsen staining

General description

Hydrochloric acid in ethanol - for microscopy, is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolatefuchsin complex in the cell wall. With the Kinyoun method, a modification of the AFB-Color carbol fuchsin solution renders heating unnecessary (cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Product number 1.00327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
Pretreatment of the specimens with Sputofluol^™ dissolves the bacteria from the surrounding viscid sputum and cell material. It is an IVD and CE registered product and can be applied for diagnostical purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Legal Information

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS02,GHS05
• signalword: Danger
• hcodes: H225 - H290 - H319
• pcodes: P210 - P233 - P234 - P240 - P241 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1
• Storage Class: 3 - Flammable liquids
• wgk: WGK 1
• flash_point_f: 69.8 °F
• flash_point_c: 21 °C