Merck
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70168

Millipore

Ox-bile, dehydrated, purified

suitable for microbiology

NACRES:
NA.85

Quality Level

sterility

non-sterile

form

powder

impurities

~5% sodium chloride

ign. residue

≤22% (as SO4)

loss

≤8% loss on drying

pH

6-8 (2% in H2O)

application(s)

microbiology

General description

Dehydrated, purified fresh bile which is used as a selective inhibitory agent in bile media. Ox-bile desiccated is prepared by a low temperature dehydration process which ensures a uniform product and eliminates the difficulties encountered in obtaining satisfactory supplies of fresh bile. An 8 to 10% solution of the dehydrated product is equivalent to fresh bile. 1 gram of dried Ox Bile is equivalent to 8 grams of fresh bile.

Application

Ox-bile, dehydrated, purified has been used:
  • as a supplement in De Man, Rogosa and Sharpe agar (MRS) broth for culturing lactic acid bacteria
  • in bile salt tolerance testing of Lactococcus lactis and Lactobacillus reuteri
  • as a component of simulated gastric and intestinal fluids to mimic bile for Mycobacterium bovis BCG vaccine survival studies

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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