Merck
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04511

Supelco

Live/Dead Cell Double Staining Kit

suitable for fluorescence

Synonym(s):
Staining kit for live/dead cells
NACRES:
NA.32

suitability

suitable for fluorescence

Quality Level

storage temp.

−20°C

Application

The Live/Dead Cell Double Staining Kit is utilized for simultaneous fluorescence staining of viable and dead cells. This kit contains calcein-AM and propidium iodide (PI) solutions, which stain viable and dead cells, respectively. Calcein-AM, acetoxymethyl ester of calcein, is highly lipophilic and cell membrane permeable. Though calcein-AM itself is not a fluorescent molecule, the calcein generated from Calcein-AM by esterase in a viable cell emits a strong green fluorescence (λex 490 nm, λem 515 nm). Therefore, calcein-AM only stains viable cells. Alternatively, the nuclei staining dye PI cannot pass through a viable cell membrane. It reaches the nucleus by passing through disordered areas of dead cell membrane, and intercalates with the DNA double helix of the cell to emit red fluorescence (λex 535 nm, λem 617 nm). Since both calcein and PI-DNA can be excited with 490 nm light, simultaneous monitoring of viable and dead cells is possible with a fluorescence microscope. Using λex 545 nm, only dead cells can be observed.

Kit Components Only

Product No.
Description

  • Solution A (Calcein AM solution) 4 × 50

  • Solution B (propidium iodide solution) 300 μL

Storage Class Code

10 - Combustible liquids

Flash Point(F)

185.0 °F - closed cup

Flash Point(C)

85 °C - closed cup

Certificate of Analysis

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Certificate of Origin

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