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Merck

94403

Hoechst 33258 solution

1 mg/mL in H2O, ≥98.0% (HPLC)

Synonym(s):

bisBenzimide H 33258 solution

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About This Item

Empirical Formula (Hill Notation):
C25H24N6O · 3HCl
CAS Number:
23491-45-4
Molecular Weight:
533.88
MDL number:
MFCD29074011
UNSPSC Code:
12171500
PubChem Substance ID:
329770334
NACRES:
NA.32

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Assay

≥98.0% (HPLC)

form

liquid

mol wt

533.88 g/mol

concentration

1 mg/mL in H2O

color

colorless
very light green-yellow to dark green-yellow
very light yellow to dark yellow

pH

6.0-8.0 (25 °C)

solubility

DMF: soluble
H2O: soluble

density

1.00 g/cm3

fluorescence

λex 355 nm; λem 465 nm in TE buffer; DNA

storage temp.

2-8°C

SMILES string

Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6

InChI

1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChI key

SMNPLAKEGAEPJD-UHFFFAOYSA-N

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This Item
14530382061B1155
Hoechst 33258 solution 1 mg/mL in H2O, ≥98.0% (HPLC)

Sigma-Aldrich

94403

Hoechst 33258 solution

bisBenzimide H 33258 suitable for fluorescence, ≥98.0% (HPLC)

Sigma-Aldrich

14530

bisBenzimide H 33258

Bisbenzimide H 33258 Fluorochrome, Trihydrochloride Membrane-permeable, adenine-thymine-specific fluorescent stain.

Sigma-Aldrich

382061

Bisbenzimide H 33258 Fluorochrome, Trihydrochloride

bisBenzimide H 33258 powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC)

Sigma-Aldrich

B1155

bisBenzimide H 33258

form

liquid

form

powder

form

solid

form

powder

assay

≥98.0% (HPLC)

assay

≥98.0% (HPLC)

assay

≥98% (HPLC)

assay

≥98% (HPLC and TLC)

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

solubility

DMF: soluble, H2O: soluble

solubility

water and ethanol: 10 mg/mL, H2O: soluble, ethanol: soluble

solubility

-

solubility

H2O: 10 mg/mL, water and ethanol: 10 mg/mL

concentration

1 mg/mL in H2O

concentration

-

concentration

-

concentration

-

General description

Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. [1]It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.[2]

Application

Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA.[3] Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.[4]

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9215
BCCN1642
BCCN0204
BCCM2644
BCCL1611

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Natalia Busto et al.
The journal of physical chemistry. B, 119(13), 4575-4581 (2015-03-12)
The functionality of the bisbenzimide Hoechst 33258 in solution has been largely exploited in the quantification of DNA. Understanding of its behavior is essential to promote its widespread application and learning of biological processes. A detailed study of the dimerization
Cornelia Fritsch et al.
PLoS genetics, 15(7), e1008269-e1008269 (2019-07-13)
Development of eye tissue is initiated by a conserved set of transcription factors termed retinal determination network (RDN). In the fruit fly Drosophila melanogaster, the zinc-finger transcription factor Glass acts directly downstream of the RDN to control identity of photoreceptor
A Fede et al.
Structure (London, England : 1993), 1(3), 177-186 (1993-11-15)
The chromosomal stain, Hoechst 33258, binds to the minor groove of the DNA double helix and specifically recognizes a run of four A-T base pairs. Extensive biochemical and biophysical studies have been aimed at understanding the binding of the dye
Michael R Green et al.
Cold Spring Harbor protocols, 2017(5) (2017-05-04)
Measuring the concentration of DNA using fluorometry is more sensitive than spectrophotometry, allowing the detection of nanogram quantities of DNA. In this assay, DNA preparations of known and unknown concentrations are incubated with the fluorochrome Hoechst 33258. Absorption values for
In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.
T R Chen
Experimental cell research, 104(2), 255-262 (1977-02-01)
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