C4951

Cholesterol-Water Soluble

powder, BioReagent, suitable for cell culture

• Synonym(s): Cholesterol–methyl-β-cyclodextrin
• NACRES: NA.75

Properties

• biological source: ovine wool
• Quality Level: 200
• product line: BioReagent
• form: powder
• technique(s): cell culture | mammalian: suitable
• solubility: H₂O: 300 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

Application

Cholesterol-Water Soluble has been used:

• in free cholesterol (FC) overloading of rat smooth muscle cells (SMCs)
• in cytotoxicity assay
• for the incubation of CD4⁺ T cells with Helicobacter pylori
• to study the membrane cholesterol level in SH-SY5Y human neuroblastoma cells
• to study the effects of cholesterol on cell viability and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction in primary cultured rat hippocampal neurons
• as a supplement for growth of bacterial culture
• to examine the interaction of S protein with angiotensin-converting enzyme 2 (ACE2)
• to investigate the effects of cholesterol on potassium currents in the inner hair cells of guinea pigs
• for reversible inhibition of proteinase K

Packaging

Package size based on cholesterol

Other Notes

Contains approx. 40 mg of cholesterol per gram; balance methyl-β-cyclodextrin.

Preparation Note

Preparea solution at 300mg/mL in water. Let thesolution sit undisturbed for one hour after diluting to obtain completesolubilization of product.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves